Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26495 and BPHYT_RS26500 are separated by 39 nucleotides BPHYT_RS26500 and BPHYT_RS26505 overlap by 4 nucleotides BPHYT_RS26505 and BPHYT_RS26510 are separated by 160 nucleotides BPHYT_RS26510 and BPHYT_RS26515 are separated by 24 nucleotides BPHYT_RS26515 and BPHYT_RS26520 overlap by 2 nucleotides BPHYT_RS26520 and BPHYT_RS26525 are separated by 26 nucleotides
BPHYT_RS26495: BPHYT_RS26495 - glutathione S-transferase, at 1,515,044 to 1,515,682
_RS26495
BPHYT_RS26500: BPHYT_RS26500 - ATPase, at 1,515,722 to 1,516,171
_RS26500
BPHYT_RS26505: BPHYT_RS26505 - ArsR family transcriptional regulator, at 1,516,168 to 1,516,503
_RS26505
BPHYT_RS26510: BPHYT_RS26510 - transcriptional regulator, at 1,516,664 to 1,517,569
_RS26510
BPHYT_RS26515: BPHYT_RS26515 - HAD family hydrolase, at 1,517,594 to 1,518,208
_RS26515
BPHYT_RS26520: BPHYT_RS26520 - hypothetical protein, at 1,518,207 to 1,518,386
_RS26520
BPHYT_RS26525: BPHYT_RS26525 - amino acid ABC transporter substrate-binding protein, at 1,518,413 to 1,519,564
_RS26525
Position (kb)
1516
1517
1518 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1515.670 kb on - strand at 1516.857 kb on - strand, within BPHYT_RS26510 at 1516.865 kb on + strand, within BPHYT_RS26510 at 1517.175 kb on - strand, within BPHYT_RS26510 at 1517.595 kb on + strand at 1517.595 kb on + strand at 1517.875 kb on + strand, within BPHYT_RS26515 at 1517.875 kb on + strand, within BPHYT_RS26515
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 1,515,670 - -1.5 1,516,857 - BPHYT_RS26510 0.21 +1.0 1,516,865 + BPHYT_RS26510 0.22 +1.7 1,517,175 - BPHYT_RS26510 0.56 +2.8 1,517,595 + +0.6 1,517,595 + -0.1 1,517,875 + BPHYT_RS26515 0.46 +0.5 1,517,875 + BPHYT_RS26515 0.46 +1.2
Or see this region's nucleotide sequence