Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25465 and BPHYT_RS25470 are separated by 26 nucleotides BPHYT_RS25470 and BPHYT_RS25475 are separated by 115 nucleotides BPHYT_RS25475 and BPHYT_RS25480 are separated by 173 nucleotides BPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotides
BPHYT_RS25465: BPHYT_RS25465 - 1,2-oxophytodienoate reductase, at 1,291,383 to 1,292,471
_RS25465
BPHYT_RS25470: BPHYT_RS25470 - alkylhydroperoxidase, at 1,292,498 to 1,293,034
_RS25470
BPHYT_RS25475: BPHYT_RS25475 - LysR family transcriptional regulator, at 1,293,150 to 1,294,064
_RS25475
BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879
_RS25480
BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750
_RS25485
Position (kb) 1293
1294
1295 Strain fitness (log2 ratio) -1
0
1
2 at 1292.208 kb on + strand, within BPHYT_RS25465 at 1292.342 kb on + strand, within BPHYT_RS25465 at 1292.476 kb on - strand at 1292.710 kb on + strand, within BPHYT_RS25470 at 1292.711 kb on - strand, within BPHYT_RS25470 at 1293.099 kb on - strand at 1293.099 kb on - strand at 1293.139 kb on + strand at 1293.293 kb on - strand, within BPHYT_RS25475 at 1294.151 kb on + strand at 1294.353 kb on + strand, within BPHYT_RS25480 at 1294.355 kb on + strand, within BPHYT_RS25480 at 1294.508 kb on - strand, within BPHYT_RS25480 at 1294.511 kb on + strand, within BPHYT_RS25480 at 1294.512 kb on - strand, within BPHYT_RS25480
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 1,292,208 + BPHYT_RS25465 0.76 +0.4 1,292,342 + BPHYT_RS25465 0.88 -0.6 1,292,476 - -1.5 1,292,710 + BPHYT_RS25470 0.39 +0.6 1,292,711 - BPHYT_RS25470 0.40 +1.5 1,293,099 - +0.4 1,293,099 - +0.6 1,293,139 + -0.0 1,293,293 - BPHYT_RS25475 0.16 -1.1 1,294,151 + -0.6 1,294,353 + BPHYT_RS25480 0.18 -0.5 1,294,355 + BPHYT_RS25480 0.18 -1.4 1,294,508 - BPHYT_RS25480 0.42 -1.6 1,294,511 + BPHYT_RS25480 0.43 -1.1 1,294,512 - BPHYT_RS25480 0.43 +1.8
Or see this region's nucleotide sequence