Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24965

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24960 and BPHYT_RS24965 are separated by 400 nucleotidesBPHYT_RS24965 and BPHYT_RS24970 are separated by 64 nucleotidesBPHYT_RS24970 and BPHYT_RS24975 are separated by 193 nucleotidesBPHYT_RS24975 and BPHYT_RS24980 overlap by 4 nucleotides BPHYT_RS24960: BPHYT_RS24960 - phospholipase C, at 1,170,988 to 1,172,754 _RS24960 BPHYT_RS24965: BPHYT_RS24965 - hypothetical protein, at 1,173,155 to 1,173,475 _RS24965 BPHYT_RS24970: BPHYT_RS24970 - hypothetical protein, at 1,173,540 to 1,173,641 _RS24970 BPHYT_RS24975: BPHYT_RS24975 - RNA polymerase sigma24 factor, at 1,173,835 to 1,174,395 _RS24975 BPHYT_RS24980: BPHYT_RS24980 - hypothetical protein, at 1,174,392 to 1,174,820 _RS24980 Position (kb) 1173 1174Strain fitness (log2 ratio) -1 0 1 2 3at 1172.195 kb on + strand, within BPHYT_RS24960at 1172.298 kb on - strand, within BPHYT_RS24960at 1172.340 kb on + strand, within BPHYT_RS24960at 1172.746 kb on - strandat 1172.826 kb on + strandat 1172.827 kb on - strandat 1172.950 kb on + strandat 1173.139 kb on - strandat 1173.363 kb on - strand, within BPHYT_RS24965at 1173.634 kb on + strandat 1173.637 kb on - strandat 1173.649 kb on + strandat 1173.649 kb on + strandat 1174.255 kb on + strand, within BPHYT_RS24975at 1174.256 kb on - strand, within BPHYT_RS24975at 1174.259 kb on - strand, within BPHYT_RS24975

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,172,195 + BPHYT_RS24960 0.68 +0.1
1,172,298 - BPHYT_RS24960 0.74 +2.0
1,172,340 + BPHYT_RS24960 0.77 +0.6
1,172,746 - -1.5
1,172,826 + +0.4
1,172,827 - -1.4
1,172,950 + +0.4
1,173,139 - +0.7
1,173,363 - BPHYT_RS24965 0.65 -1.4
1,173,634 + -0.1
1,173,637 - +0.5
1,173,649 + +0.5
1,173,649 + +0.6
1,174,255 + BPHYT_RS24975 0.75 -0.4
1,174,256 - BPHYT_RS24975 0.75 +3.7
1,174,259 - BPHYT_RS24975 0.76 +0.4

Or see this region's nucleotide sequence