Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24660 and BPHYT_RS24665 are separated by 35 nucleotides BPHYT_RS24665 and BPHYT_RS24670 overlap by 4 nucleotides BPHYT_RS24670 and BPHYT_RS24675 overlap by 4 nucleotides
BPHYT_RS24660: BPHYT_RS24660 - ABC transporter ATP-binding protein, at 1,102,382 to 1,103,455
_RS24660
BPHYT_RS24665: BPHYT_RS24665 - ABC transporter permease, at 1,103,491 to 1,104,378
_RS24665
BPHYT_RS24670: BPHYT_RS24670 - ABC transporter permease, at 1,104,375 to 1,105,334
_RS24670
BPHYT_RS24675: BPHYT_RS24675 - spermidine/putrescine ABC transporter substrate-binding protein, at 1,105,331 to 1,106,425
_RS24675
Position (kb) 1104
1105
1106 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1103.444 kb on + strand at 1104.024 kb on - strand, within BPHYT_RS24665 at 1104.615 kb on + strand, within BPHYT_RS24670 at 1104.796 kb on + strand, within BPHYT_RS24670 at 1105.353 kb on + strand at 1105.635 kb on + strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.696 kb on - strand, within BPHYT_RS24675 at 1105.932 kb on + strand, within BPHYT_RS24675 at 1105.968 kb on + strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1106.249 kb on - strand, within BPHYT_RS24675
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 1,103,444 + -1.5 1,104,024 - BPHYT_RS24665 0.60 +0.3 1,104,615 + BPHYT_RS24670 0.25 -1.1 1,104,796 + BPHYT_RS24670 0.44 -1.6 1,105,353 + -0.5 1,105,635 + BPHYT_RS24675 0.28 +1.0 1,105,636 - BPHYT_RS24675 0.28 +2.7 1,105,636 - BPHYT_RS24675 0.28 -0.2 1,105,696 - BPHYT_RS24675 0.33 +0.3 1,105,932 + BPHYT_RS24675 0.55 -1.3 1,105,968 + BPHYT_RS24675 0.58 -1.8 1,105,969 - BPHYT_RS24675 0.58 -1.4 1,105,969 - BPHYT_RS24675 0.58 -0.4 1,106,249 - BPHYT_RS24675 0.84 -0.8
Or see this region's nucleotide sequence