Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23590 and BPHYT_RS23595 are separated by 395 nucleotides BPHYT_RS23595 and BPHYT_RS23600 are separated by 250 nucleotides BPHYT_RS23600 and BPHYT_RS23605 are separated by 73 nucleotides BPHYT_RS23605 and BPHYT_RS23610 are separated by 45 nucleotides
BPHYT_RS23590: BPHYT_RS23590 - hypothetical protein, at 866,359 to 867,585
_RS23590
BPHYT_RS23595: BPHYT_RS23595 - hypothetical protein, at 867,981 to 868,166
_RS23595
BPHYT_RS23600: BPHYT_RS23600 - hypothetical protein, at 868,417 to 868,695
_RS23600
BPHYT_RS23605: BPHYT_RS23605 - hypothetical protein, at 868,769 to 869,050
_RS23605
BPHYT_RS23610: BPHYT_RS23610 - alpha/beta hydrolase, at 869,096 to 870,091
_RS23610
Position (kb) 868
869 Strain fitness (log2 ratio) -2
-1
0
1 at 867.431 kb on - strand, within BPHYT_RS23590 at 867.431 kb on - strand, within BPHYT_RS23590 at 867.552 kb on - strand at 867.559 kb on + strand at 867.560 kb on - strand at 867.681 kb on + strand at 867.681 kb on + strand at 868.332 kb on + strand at 868.332 kb on + strand at 868.436 kb on + strand at 868.627 kb on + strand, within BPHYT_RS23600 at 868.640 kb on - strand, within BPHYT_RS23600 at 868.642 kb on + strand, within BPHYT_RS23600 at 868.682 kb on - strand at 868.868 kb on + strand, within BPHYT_RS23605 at 868.868 kb on + strand, within BPHYT_RS23605 at 868.868 kb on + strand, within BPHYT_RS23605 at 868.868 kb on + strand, within BPHYT_RS23605 at 868.869 kb on - strand, within BPHYT_RS23605 at 869.097 kb on + strand at 869.172 kb on + strand at 869.172 kb on + strand at 869.173 kb on - strand at 869.303 kb on + strand, within BPHYT_RS23610 at 869.423 kb on - strand, within BPHYT_RS23610 at 869.423 kb on - strand, within BPHYT_RS23610
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 867,431 - BPHYT_RS23590 0.87 -0.1 867,431 - BPHYT_RS23590 0.87 -1.4 867,552 - +0.4 867,559 + +0.7 867,560 - +0.4 867,681 + -0.6 867,681 + -0.9 868,332 + -0.4 868,332 + +0.6 868,436 + -2.5 868,627 + BPHYT_RS23600 0.75 -1.6 868,640 - BPHYT_RS23600 0.80 -1.2 868,642 + BPHYT_RS23600 0.81 -1.3 868,682 - +0.4 868,868 + BPHYT_RS23605 0.35 +0.4 868,868 + BPHYT_RS23605 0.35 -0.3 868,868 + BPHYT_RS23605 0.35 -0.3 868,868 + BPHYT_RS23605 0.35 -1.4 868,869 - BPHYT_RS23605 0.35 +0.2 869,097 + -0.1 869,172 + -0.0 869,172 + +0.8 869,173 - -0.3 869,303 + BPHYT_RS23610 0.21 +1.1 869,423 - BPHYT_RS23610 0.33 -0.7 869,423 - BPHYT_RS23610 0.33 -0.1
Or see this region's nucleotide sequence