Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23000

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22995 and BPHYT_RS23000 are separated by 28 nucleotidesBPHYT_RS23000 and BPHYT_RS23005 are separated by 765 nucleotides BPHYT_RS22995: BPHYT_RS22995 - porin, at 719,141 to 720,247 _RS22995 BPHYT_RS23000: BPHYT_RS23000 - molybdopterin-dependent oxidoreductase, at 720,276 to 720,872 _RS23000 BPHYT_RS23005: BPHYT_RS23005 - carboxymuconolactone decarboxylase, at 721,638 to 722,186 _RS23005 Position (kb) 720 721Strain fitness (log2 ratio) -1 0 1at 719.294 kb on + strand, within BPHYT_RS22995at 719.550 kb on + strand, within BPHYT_RS22995at 719.551 kb on - strand, within BPHYT_RS22995at 719.551 kb on - strand, within BPHYT_RS22995at 719.741 kb on + strand, within BPHYT_RS22995at 719.741 kb on + strand, within BPHYT_RS22995at 719.850 kb on + strand, within BPHYT_RS22995at 720.101 kb on + strand, within BPHYT_RS22995at 720.102 kb on - strand, within BPHYT_RS22995at 720.973 kb on + strandat 721.420 kb on + strandat 721.544 kb on + strandat 721.545 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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719,294 + BPHYT_RS22995 0.14 -1.3
719,550 + BPHYT_RS22995 0.37 -0.0
719,551 - BPHYT_RS22995 0.37 +0.2
719,551 - BPHYT_RS22995 0.37 -0.4
719,741 + BPHYT_RS22995 0.54 +0.0
719,741 + BPHYT_RS22995 0.54 +0.0
719,850 + BPHYT_RS22995 0.64 -0.4
720,101 + BPHYT_RS22995 0.87 +1.2
720,102 - BPHYT_RS22995 0.87 +0.4
720,973 + +0.6
721,420 + -0.8
721,544 + +0.5
721,545 - -0.8

Or see this region's nucleotide sequence