Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21850 and BPHYT_RS21855 are separated by 98 nucleotides BPHYT_RS21855 and BPHYT_RS21860 are separated by 778 nucleotides
BPHYT_RS21850: BPHYT_RS21850 - pyrimidine reductase, at 456,173 to 456,850
_RS21850
BPHYT_RS21855: BPHYT_RS21855 - hypothetical protein, at 456,949 to 457,716
_RS21855
BPHYT_RS21860: BPHYT_RS21860 - hypothetical protein, at 458,495 to 458,953
_RS21860
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1
2 at 456.182 kb on + strand at 456.183 kb on - strand at 456.184 kb on + strand at 456.184 kb on + strand at 456.184 kb on + strand at 456.185 kb on - strand at 456.185 kb on - strand at 456.264 kb on - strand, within BPHYT_RS21850 at 456.264 kb on - strand, within BPHYT_RS21850 at 456.416 kb on + strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 457.227 kb on - strand, within BPHYT_RS21855 at 457.285 kb on - strand, within BPHYT_RS21855 at 457.285 kb on - strand, within BPHYT_RS21855 at 457.559 kb on + strand, within BPHYT_RS21855 at 457.560 kb on - strand, within BPHYT_RS21855 at 457.560 kb on - strand at 457.560 kb on - strand, within BPHYT_RS21855 at 457.787 kb on + strand at 457.788 kb on - strand at 457.879 kb on - strand at 457.914 kb on + strand at 458.069 kb on + strand at 458.074 kb on - strand at 458.199 kb on - strand at 458.203 kb on + strand at 458.203 kb on + strand at 458.204 kb on - strand at 458.323 kb on + strand at 458.324 kb on - strand at 458.444 kb on + strand at 458.445 kb on - strand at 458.497 kb on - strand at 458.653 kb on - strand, within BPHYT_RS21860
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 456,182 + -1.6 456,183 - +1.2 456,184 + +1.3 456,184 + -2.2 456,184 + +1.0 456,185 - +0.9 456,185 - -1.1 456,264 - BPHYT_RS21850 0.13 +0.9 456,264 - BPHYT_RS21850 0.13 +0.3 456,416 + BPHYT_RS21850 0.36 +0.8 456,528 - BPHYT_RS21850 0.52 +0.4 456,528 - BPHYT_RS21850 0.52 -0.1 456,528 - BPHYT_RS21850 0.52 -1.4 457,227 - BPHYT_RS21855 0.36 -1.7 457,285 - BPHYT_RS21855 0.44 -1.9 457,285 - BPHYT_RS21855 0.44 -0.6 457,559 + BPHYT_RS21855 0.79 -1.4 457,560 - BPHYT_RS21855 0.80 +0.1 457,560 - +0.6 457,560 - BPHYT_RS21855 0.80 -1.4 457,787 + -1.2 457,788 - -0.0 457,879 - +1.2 457,914 + -1.7 458,069 + +1.9 458,074 - +1.4 458,199 - -1.3 458,203 + +0.5 458,203 + +1.5 458,204 - -1.1 458,323 + -2.6 458,324 - -0.1 458,444 + -0.7 458,445 - -1.4 458,497 - +1.1 458,653 - BPHYT_RS21860 0.34 +0.6
Or see this region's nucleotide sequence