Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21050 and BPHYT_RS21055 are separated by 2 nucleotides BPHYT_RS21055 and BPHYT_RS21060 are separated by 202 nucleotides
BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900
_RS21050
BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733
_RS21055
BPHYT_RS21060: BPHYT_RS21060 - hypothetical protein, at 284,936 to 286,408
_RS21060
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 282.921 kb on + strand at 282.970 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 283.080 kb on + strand at 283.297 kb on + strand at 283.301 kb on - strand at 283.304 kb on - strand at 283.398 kb on + strand, within BPHYT_RS21050 at 283.398 kb on + strand, within BPHYT_RS21050 at 283.474 kb on + strand, within BPHYT_RS21050 at 283.632 kb on - strand, within BPHYT_RS21050 at 283.831 kb on - strand, within BPHYT_RS21050 at 284.007 kb on + strand, within BPHYT_RS21055 at 284.131 kb on - strand, within BPHYT_RS21055 at 284.264 kb on - strand, within BPHYT_RS21055 at 284.497 kb on + strand, within BPHYT_RS21055 at 284.668 kb on - strand at 284.668 kb on - strand at 284.675 kb on - strand at 284.810 kb on - strand at 285.011 kb on - strand at 285.257 kb on + strand, within BPHYT_RS21060 at 285.262 kb on - strand, within BPHYT_RS21060 at 285.294 kb on + strand, within BPHYT_RS21060 at 285.295 kb on - strand, within BPHYT_RS21060
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 282,921 + -0.2 282,970 + +0.1 282,979 + +0.7 282,979 + -0.5 282,979 + +0.2 283,080 + -1.3 283,297 + -0.1 283,301 - +3.9 283,304 - -0.2 283,398 + BPHYT_RS21050 0.13 -1.6 283,398 + BPHYT_RS21050 0.13 -0.6 283,474 + BPHYT_RS21050 0.26 -1.9 283,632 - BPHYT_RS21050 0.53 +2.2 283,831 - BPHYT_RS21050 0.88 +0.3 284,007 + BPHYT_RS21055 0.13 -1.1 284,131 - BPHYT_RS21055 0.27 +2.9 284,264 - BPHYT_RS21055 0.43 -1.5 284,497 + BPHYT_RS21055 0.71 -1.8 284,668 - -0.6 284,668 - +0.1 284,675 - -1.3 284,810 - +1.2 285,011 - -1.2 285,257 + BPHYT_RS21060 0.22 +0.6 285,262 - BPHYT_RS21060 0.22 -0.5 285,294 + BPHYT_RS21060 0.24 -0.0 285,295 - BPHYT_RS21060 0.24 +1.1
Or see this region's nucleotide sequence