Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20130 and BPHYT_RS20135 are separated by 196 nucleotides BPHYT_RS20135 and BPHYT_RS20140 are separated by 37 nucleotides BPHYT_RS20140 and BPHYT_RS20145 overlap by 4 nucleotides BPHYT_RS20145 and BPHYT_RS20150 are separated by 77 nucleotides
BPHYT_RS20130: BPHYT_RS20130 - diguanylate phosphodiesterase, at 59,338 to 60,105
_RS20130
BPHYT_RS20135: BPHYT_RS20135 - UDP-N-acetyl-D-mannosamine transferase, at 60,302 to 61,060
_RS20135
BPHYT_RS20140: BPHYT_RS20140 - hypothetical protein, at 61,098 to 62,264
_RS20140
BPHYT_RS20145: BPHYT_RS20145 - hypothetical protein, at 62,261 to 62,977
_RS20145
BPHYT_RS20150: BPHYT_RS20150 - glycosyl transferase family 1, at 63,055 to 64,146
_RS20150
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1
2 at 60.135 kb on - strand at 60.135 kb on - strand at 60.401 kb on - strand, within BPHYT_RS20135 at 60.628 kb on + strand, within BPHYT_RS20135 at 60.855 kb on - strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135 at 61.139 kb on - strand at 61.232 kb on - strand, within BPHYT_RS20140 at 61.311 kb on - strand, within BPHYT_RS20140 at 61.408 kb on + strand, within BPHYT_RS20140 at 61.437 kb on - strand, within BPHYT_RS20140 at 61.699 kb on + strand, within BPHYT_RS20140 at 61.700 kb on - strand, within BPHYT_RS20140 at 61.769 kb on + strand, within BPHYT_RS20140 at 62.160 kb on + strand at 62.161 kb on - strand at 62.592 kb on + strand, within BPHYT_RS20145 at 63.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 60,135 - -0.7 60,135 - +1.9 60,401 - BPHYT_RS20135 0.13 -0.2 60,628 + BPHYT_RS20135 0.43 +0.9 60,855 - BPHYT_RS20135 0.73 +1.9 60,875 + BPHYT_RS20135 0.75 -1.2 60,875 + BPHYT_RS20135 0.75 -0.3 61,139 - +0.2 61,232 - BPHYT_RS20140 0.11 +0.8 61,311 - BPHYT_RS20140 0.18 -0.9 61,408 + BPHYT_RS20140 0.27 -1.5 61,437 - BPHYT_RS20140 0.29 +0.2 61,699 + BPHYT_RS20140 0.51 -1.2 61,700 - BPHYT_RS20140 0.52 -0.1 61,769 + BPHYT_RS20140 0.57 -0.9 62,160 + +2.3 62,161 - +0.1 62,592 + BPHYT_RS20145 0.46 +0.1 63,093 - +1.7
Or see this region's nucleotide sequence