Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19930 and BPHYT_RS19935 are separated by 24 nucleotides BPHYT_RS19935 and BPHYT_RS19940 are separated by 5 nucleotides BPHYT_RS19940 and BPHYT_RS19945 are separated by 470 nucleotides
BPHYT_RS19930: BPHYT_RS19930 - transcriptional regulator, at 17,606 to 18,961
_RS19930
BPHYT_RS19935: BPHYT_RS19935 - protein serine/threonine phosphatase, at 18,986 to 19,756
_RS19935
BPHYT_RS19940: BPHYT_RS19940 - hypothetical protein, at 19,762 to 20,049
_RS19940
BPHYT_RS19945: BPHYT_RS19945 - hypothetical protein, at 20,520 to 21,017
_RS19945
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1 at 19.413 kb on + strand, within BPHYT_RS19935 at 19.469 kb on + strand, within BPHYT_RS19935 at 19.770 kb on + strand at 19.831 kb on + strand, within BPHYT_RS19940 at 19.832 kb on - strand, within BPHYT_RS19940 at 19.871 kb on + strand, within BPHYT_RS19940 at 19.934 kb on + strand, within BPHYT_RS19940 at 19.998 kb on + strand, within BPHYT_RS19940 at 20.040 kb on + strand at 20.041 kb on - strand at 20.106 kb on + strand at 20.107 kb on - strand at 20.120 kb on - strand at 20.120 kb on - strand at 20.643 kb on + strand, within BPHYT_RS19945 at 20.670 kb on + strand, within BPHYT_RS19945 at 20.778 kb on + strand, within BPHYT_RS19945
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 19,413 + BPHYT_RS19935 0.55 -0.3 19,469 + BPHYT_RS19935 0.63 +0.3 19,770 + -0.7 19,831 + BPHYT_RS19940 0.24 +0.3 19,832 - BPHYT_RS19940 0.24 +0.7 19,871 + BPHYT_RS19940 0.38 +0.9 19,934 + BPHYT_RS19940 0.60 +0.2 19,998 + BPHYT_RS19940 0.82 -1.6 20,040 + -1.4 20,041 - -0.6 20,106 + -0.2 20,107 - +0.5 20,120 - -2.2 20,120 - +0.2 20,643 + BPHYT_RS19945 0.25 -1.0 20,670 + BPHYT_RS19945 0.30 -0.4 20,778 + BPHYT_RS19945 0.52 +0.5
Or see this region's nucleotide sequence