Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17065 and BPHYT_RS17070 overlap by 4 nucleotides BPHYT_RS17070 and BPHYT_RS17075 are separated by 9 nucleotides BPHYT_RS17075 and BPHYT_RS17080 are separated by 81 nucleotides
BPHYT_RS17065: BPHYT_RS17065 - N-acetyl-anhydromuranmyl-L-alanine amidase, at 3,869,854 to 3,870,450
_RS17065
BPHYT_RS17070: BPHYT_RS17070 - pyrimidine deaminase, at 3,870,447 to 3,870,770
_RS17070
BPHYT_RS17075: BPHYT_RS17075 - ABC transporter permease, at 3,870,780 to 3,871,709
_RS17075
BPHYT_RS17080: BPHYT_RS17080 - signal recognition particle protein, at 3,871,791 to 3,873,158
_RS17080
Position (kb)
3870
3871
3872 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3870.422 kb on + strand at 3870.760 kb on - strand at 3870.787 kb on - strand at 3870.833 kb on - strand at 3870.833 kb on - strand at 3871.020 kb on + strand, within BPHYT_RS17075 at 3871.020 kb on + strand, within BPHYT_RS17075 at 3871.021 kb on - strand, within BPHYT_RS17075 at 3871.403 kb on - strand, within BPHYT_RS17075 at 3871.403 kb on - strand, within BPHYT_RS17075 at 3871.484 kb on + strand, within BPHYT_RS17075 at 3871.694 kb on - strand at 3871.694 kb on - strand at 3871.698 kb on + strand at 3871.698 kb on + strand at 3871.698 kb on + strand at 3871.698 kb on + strand at 3871.699 kb on - strand at 3871.699 kb on - strand at 3871.720 kb on + strand at 3871.725 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 3,870,422 + +1.2 3,870,760 - -0.1 3,870,787 - -3.1 3,870,833 - +0.3 3,870,833 - -0.5 3,871,020 + BPHYT_RS17075 0.26 +0.6 3,871,020 + BPHYT_RS17075 0.26 +0.0 3,871,021 - BPHYT_RS17075 0.26 +0.1 3,871,403 - BPHYT_RS17075 0.67 -0.1 3,871,403 - BPHYT_RS17075 0.67 +1.1 3,871,484 + BPHYT_RS17075 0.76 +1.6 3,871,694 - -1.3 3,871,694 - +0.4 3,871,698 + +0.4 3,871,698 + -0.6 3,871,698 + +2.0 3,871,698 + +0.3 3,871,699 - +1.3 3,871,699 - -1.8 3,871,720 + -2.7 3,871,725 + +3.2
Or see this region's nucleotide sequence