Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16690 and BPHYT_RS16695 overlap by 1 nucleotides BPHYT_RS16695 and BPHYT_RS16700 are separated by 82 nucleotides BPHYT_RS16700 and BPHYT_RS16705 are separated by 285 nucleotides
BPHYT_RS16690: BPHYT_RS16690 - glutamate/aspartate transporter permease GltK, at 3,778,539 to 3,779,216
_RS16690
BPHYT_RS16695: BPHYT_RS16695 - glutamate/aspartate ABC transporter permease GltJ, at 3,779,216 to 3,779,956
_RS16695
BPHYT_RS16700: BPHYT_RS16700 - ABC transporter, at 3,780,039 to 3,780,932
_RS16700
BPHYT_RS16705: BPHYT_RS16705 - glutamate dehydrogenase, at 3,781,218 to 3,782,531
_RS16705
Position (kb)
3780
3781 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3779.041 kb on + strand, within BPHYT_RS16690 at 3779.041 kb on + strand, within BPHYT_RS16690 at 3779.041 kb on + strand, within BPHYT_RS16690 at 3779.174 kb on + strand at 3779.217 kb on + strand at 3779.218 kb on - strand at 3779.218 kb on - strand at 3779.218 kb on - strand at 3779.218 kb on - strand at 3779.232 kb on + strand at 3779.232 kb on + strand at 3779.301 kb on + strand, within BPHYT_RS16695 at 3779.302 kb on - strand, within BPHYT_RS16695 at 3779.302 kb on - strand, within BPHYT_RS16695 at 3779.332 kb on - strand, within BPHYT_RS16695 at 3779.332 kb on - strand, within BPHYT_RS16695 at 3779.332 kb on - strand, within BPHYT_RS16695 at 3779.347 kb on - strand, within BPHYT_RS16695 at 3779.423 kb on - strand, within BPHYT_RS16695 at 3779.423 kb on - strand, within BPHYT_RS16695 at 3779.485 kb on - strand, within BPHYT_RS16695 at 3779.485 kb on - strand, within BPHYT_RS16695 at 3779.700 kb on + strand, within BPHYT_RS16695 at 3779.731 kb on + strand, within BPHYT_RS16695 at 3779.732 kb on - strand, within BPHYT_RS16695 at 3779.732 kb on - strand, within BPHYT_RS16695 at 3779.751 kb on + strand, within BPHYT_RS16695 at 3779.752 kb on - strand, within BPHYT_RS16695 at 3779.752 kb on - strand, within BPHYT_RS16695 at 3779.752 kb on - strand, within BPHYT_RS16695 at 3779.752 kb on - strand, within BPHYT_RS16695 at 3779.752 kb on - strand, within BPHYT_RS16695 at 3779.855 kb on - strand, within BPHYT_RS16695 at 3779.893 kb on - strand at 3779.893 kb on - strand at 3779.893 kb on - strand at 3779.893 kb on - strand at 3779.949 kb on + strand at 3780.033 kb on + strand at 3780.033 kb on + strand at 3780.071 kb on - strand at 3780.071 kb on - strand at 3780.071 kb on - strand at 3780.071 kb on - strand at 3780.151 kb on + strand, within BPHYT_RS16700 at 3780.151 kb on + strand, within BPHYT_RS16700 at 3780.781 kb on + strand, within BPHYT_RS16700 at 3780.781 kb on + strand, within BPHYT_RS16700 at 3780.782 kb on - strand, within BPHYT_RS16700 at 3780.785 kb on - strand, within BPHYT_RS16700 at 3780.855 kb on - strand at 3780.967 kb on - strand at 3780.974 kb on + strand at 3780.978 kb on + strand at 3780.989 kb on + strand at 3780.989 kb on + strand at 3780.989 kb on + strand at 3780.989 kb on + strand at 3780.989 kb on + strand at 3780.990 kb on - strand at 3780.995 kb on + strand at 3780.995 kb on + strand at 3780.995 kb on + strand at 3780.996 kb on - strand at 3780.997 kb on + strand at 3780.997 kb on + strand at 3780.997 kb on + strand at 3780.997 kb on + strand at 3780.997 kb on + strand at 3780.998 kb on - strand at 3781.069 kb on - strand at 3781.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 3,779,041 + BPHYT_RS16690 0.74 -1.1 3,779,041 + BPHYT_RS16690 0.74 -1.5 3,779,041 + BPHYT_RS16690 0.74 -0.1 3,779,174 + -0.9 3,779,217 + +1.3 3,779,218 - +0.3 3,779,218 - -1.5 3,779,218 - -0.1 3,779,218 - -0.8 3,779,232 + -0.8 3,779,232 + -0.6 3,779,301 + BPHYT_RS16695 0.11 +0.2 3,779,302 - BPHYT_RS16695 0.12 -1.8 3,779,302 - BPHYT_RS16695 0.12 +0.2 3,779,332 - BPHYT_RS16695 0.16 -0.1 3,779,332 - BPHYT_RS16695 0.16 +0.3 3,779,332 - BPHYT_RS16695 0.16 -3.5 3,779,347 - BPHYT_RS16695 0.18 +0.1 3,779,423 - BPHYT_RS16695 0.28 -2.2 3,779,423 - BPHYT_RS16695 0.28 -1.0 3,779,485 - BPHYT_RS16695 0.36 -0.1 3,779,485 - BPHYT_RS16695 0.36 -0.3 3,779,700 + BPHYT_RS16695 0.65 +0.0 3,779,731 + BPHYT_RS16695 0.70 +0.6 3,779,732 - BPHYT_RS16695 0.70 -0.4 3,779,732 - BPHYT_RS16695 0.70 -1.7 3,779,751 + BPHYT_RS16695 0.72 -1.1 3,779,752 - BPHYT_RS16695 0.72 +0.1 3,779,752 - BPHYT_RS16695 0.72 -0.9 3,779,752 - BPHYT_RS16695 0.72 -0.2 3,779,752 - BPHYT_RS16695 0.72 +0.1 3,779,752 - BPHYT_RS16695 0.72 -0.7 3,779,855 - BPHYT_RS16695 0.86 +0.1 3,779,893 - -0.1 3,779,893 - -1.4 3,779,893 - -1.0 3,779,893 - -0.6 3,779,949 + +1.7 3,780,033 + +0.6 3,780,033 + -1.6 3,780,071 - -0.4 3,780,071 - +1.6 3,780,071 - -0.5 3,780,071 - -0.3 3,780,151 + BPHYT_RS16700 0.13 -0.2 3,780,151 + BPHYT_RS16700 0.13 -1.2 3,780,781 + BPHYT_RS16700 0.83 -1.8 3,780,781 + BPHYT_RS16700 0.83 -0.6 3,780,782 - BPHYT_RS16700 0.83 -1.9 3,780,785 - BPHYT_RS16700 0.83 -0.1 3,780,855 - -0.6 3,780,967 - -1.0 3,780,974 + +0.1 3,780,978 + -0.2 3,780,989 + -0.5 3,780,989 + +0.2 3,780,989 + +0.5 3,780,989 + -1.2 3,780,989 + -0.3 3,780,990 - -1.6 3,780,995 + +0.3 3,780,995 + -0.5 3,780,995 + +0.7 3,780,996 - -0.5 3,780,997 + +0.4 3,780,997 + -1.3 3,780,997 + +1.2 3,780,997 + -1.2 3,780,997 + -0.7 3,780,998 - +0.3 3,781,069 - +1.0 3,781,205 - -1.2
Or see this region's nucleotide sequence