Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15510 and BPHYT_RS15515 are separated by 136 nucleotides BPHYT_RS15515 and BPHYT_RS15520 overlap by 1 nucleotides BPHYT_RS15520 and BPHYT_RS15525 overlap by 4 nucleotides BPHYT_RS15525 and BPHYT_RS15530 are separated by 7 nucleotides
BPHYT_RS15510: BPHYT_RS15510 - pyruvate dehydrogenase, at 3,518,512 to 3,520,236
_RS15510
BPHYT_RS15515: BPHYT_RS15515 - D-aminopeptidase, at 3,520,373 to 3,521,206
_RS15515
BPHYT_RS15520: BPHYT_RS15520 - D-aminopeptidase, at 3,521,206 to 3,522,306
_RS15520
BPHYT_RS15525: BPHYT_RS15525 - glutathione ABC transporter permease GsiD, at 3,522,303 to 3,523,208
_RS15525
BPHYT_RS15530: BPHYT_RS15530 - glutathione ABC transporter permease, at 3,523,216 to 3,524,136
_RS15530
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3520.212 kb on - strand at 3520.794 kb on + strand, within BPHYT_RS15515 at 3520.794 kb on + strand, within BPHYT_RS15515 at 3520.862 kb on - strand, within BPHYT_RS15515 at 3521.224 kb on + strand at 3521.225 kb on - strand at 3521.423 kb on + strand, within BPHYT_RS15520 at 3521.656 kb on - strand, within BPHYT_RS15520 at 3521.688 kb on + strand, within BPHYT_RS15520 at 3521.732 kb on + strand, within BPHYT_RS15520 at 3521.733 kb on - strand, within BPHYT_RS15520 at 3521.733 kb on - strand, within BPHYT_RS15520 at 3521.736 kb on + strand, within BPHYT_RS15520 at 3521.737 kb on - strand, within BPHYT_RS15520 at 3521.855 kb on + strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.909 kb on + strand, within BPHYT_RS15520 at 3522.545 kb on - strand, within BPHYT_RS15525 at 3522.633 kb on - strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.635 kb on - strand, within BPHYT_RS15525 at 3522.832 kb on + strand, within BPHYT_RS15525 at 3522.835 kb on + strand, within BPHYT_RS15525 at 3522.836 kb on - strand, within BPHYT_RS15525 at 3523.250 kb on + strand at 3523.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 3,520,212 - +1.3 3,520,794 + BPHYT_RS15515 0.50 +0.7 3,520,794 + BPHYT_RS15515 0.50 -0.6 3,520,862 - BPHYT_RS15515 0.59 -0.8 3,521,224 + +0.2 3,521,225 - -1.1 3,521,423 + BPHYT_RS15520 0.20 -0.6 3,521,656 - BPHYT_RS15520 0.41 -0.3 3,521,688 + BPHYT_RS15520 0.44 +0.1 3,521,732 + BPHYT_RS15520 0.48 +0.5 3,521,733 - BPHYT_RS15520 0.48 +0.6 3,521,733 - BPHYT_RS15520 0.48 -1.4 3,521,736 + BPHYT_RS15520 0.48 -0.2 3,521,737 - BPHYT_RS15520 0.48 -2.7 3,521,855 + BPHYT_RS15520 0.59 +1.0 3,521,856 - BPHYT_RS15520 0.59 -2.3 3,521,856 - BPHYT_RS15520 0.59 -1.3 3,521,856 - BPHYT_RS15520 0.59 -1.1 3,521,909 + BPHYT_RS15520 0.64 -0.0 3,522,545 - BPHYT_RS15525 0.27 +2.7 3,522,633 - BPHYT_RS15525 0.36 -0.2 3,522,634 + BPHYT_RS15525 0.37 -1.5 3,522,634 + BPHYT_RS15525 0.37 +1.2 3,522,634 + BPHYT_RS15525 0.37 -0.1 3,522,634 + BPHYT_RS15525 0.37 -2.5 3,522,634 + BPHYT_RS15525 0.37 +0.9 3,522,635 - BPHYT_RS15525 0.37 +0.5 3,522,832 + BPHYT_RS15525 0.58 +0.1 3,522,835 + BPHYT_RS15525 0.59 +1.0 3,522,836 - BPHYT_RS15525 0.59 +0.4 3,523,250 + -0.1 3,523,250 + -0.2
Or see this region's nucleotide sequence