Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15145 and BPHYT_RS15150 overlap by 4 nucleotides BPHYT_RS15150 and BPHYT_RS15155 overlap by 1 nucleotides BPHYT_RS15155 and BPHYT_RS15160 overlap by 13 nucleotides
BPHYT_RS15145: BPHYT_RS15145 - 3-oxoacyl-ACP synthase, at 3,445,513 to 3,446,694
_RS15145
BPHYT_RS15150: BPHYT_RS15150 - membrane protein, at 3,446,691 to 3,449,102
_RS15150
BPHYT_RS15155: BPHYT_RS15155 - hypothetical protein, at 3,449,102 to 3,449,788
_RS15155
BPHYT_RS15160: BPHYT_RS15160 - hypothetical protein, at 3,449,776 to 3,450,207
_RS15160
Position (kb)
3446
3447
3448
3449
3450 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3445.856 kb on + strand, within BPHYT_RS15145 at 3446.346 kb on - strand, within BPHYT_RS15145 at 3446.575 kb on - strand, within BPHYT_RS15145 at 3446.590 kb on - strand at 3446.676 kb on - strand at 3446.676 kb on - strand at 3446.917 kb on + strand at 3447.305 kb on - strand, within BPHYT_RS15150 at 3447.335 kb on + strand, within BPHYT_RS15150 at 3447.523 kb on + strand, within BPHYT_RS15150 at 3447.574 kb on - strand, within BPHYT_RS15150 at 3447.924 kb on - strand, within BPHYT_RS15150 at 3448.084 kb on + strand, within BPHYT_RS15150 at 3448.348 kb on + strand, within BPHYT_RS15150 at 3448.398 kb on + strand, within BPHYT_RS15150 at 3448.431 kb on + strand, within BPHYT_RS15150 at 3448.485 kb on + strand, within BPHYT_RS15150 at 3448.485 kb on + strand, within BPHYT_RS15150 at 3448.486 kb on - strand, within BPHYT_RS15150 at 3448.488 kb on + strand, within BPHYT_RS15150 at 3448.489 kb on - strand, within BPHYT_RS15150 at 3448.640 kb on - strand, within BPHYT_RS15150 at 3448.744 kb on + strand, within BPHYT_RS15150 at 3448.744 kb on + strand, within BPHYT_RS15150 at 3448.745 kb on - strand, within BPHYT_RS15150 at 3448.745 kb on - strand, within BPHYT_RS15150 at 3448.769 kb on - strand, within BPHYT_RS15150 at 3448.916 kb on + strand at 3449.018 kb on - strand at 3449.179 kb on + strand, within BPHYT_RS15155 at 3449.338 kb on - strand, within BPHYT_RS15155 at 3449.338 kb on - strand, within BPHYT_RS15155 at 3449.349 kb on - strand, within BPHYT_RS15155 at 3449.479 kb on - strand, within BPHYT_RS15155 at 3449.479 kb on - strand, within BPHYT_RS15155 at 3449.491 kb on - strand, within BPHYT_RS15155 at 3449.701 kb on + strand, within BPHYT_RS15155 at 3449.732 kb on + strand at 3449.732 kb on + strand at 3449.732 kb on + strand at 3449.733 kb on - strand at 3449.892 kb on + strand, within BPHYT_RS15160 at 3449.923 kb on + strand, within BPHYT_RS15160 at 3449.982 kb on - strand, within BPHYT_RS15160 at 3449.982 kb on - strand, within BPHYT_RS15160 at 3449.999 kb on + strand, within BPHYT_RS15160 at 3450.062 kb on + strand, within BPHYT_RS15160
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 3,445,856 + BPHYT_RS15145 0.29 +2.2 3,446,346 - BPHYT_RS15145 0.70 +0.7 3,446,575 - BPHYT_RS15145 0.90 +3.9 3,446,590 - +2.1 3,446,676 - +2.7 3,446,676 - +1.9 3,446,917 + +2.5 3,447,305 - BPHYT_RS15150 0.25 +3.0 3,447,335 + BPHYT_RS15150 0.27 +2.8 3,447,523 + BPHYT_RS15150 0.34 +1.3 3,447,574 - BPHYT_RS15150 0.37 +1.6 3,447,924 - BPHYT_RS15150 0.51 +1.9 3,448,084 + BPHYT_RS15150 0.58 +2.0 3,448,348 + BPHYT_RS15150 0.69 +1.2 3,448,398 + BPHYT_RS15150 0.71 +1.4 3,448,431 + BPHYT_RS15150 0.72 +2.7 3,448,485 + BPHYT_RS15150 0.74 +2.4 3,448,485 + BPHYT_RS15150 0.74 +2.0 3,448,486 - BPHYT_RS15150 0.74 +1.7 3,448,488 + BPHYT_RS15150 0.75 +3.0 3,448,489 - BPHYT_RS15150 0.75 +1.6 3,448,640 - BPHYT_RS15150 0.81 +2.3 3,448,744 + BPHYT_RS15150 0.85 +1.5 3,448,744 + BPHYT_RS15150 0.85 +2.6 3,448,745 - BPHYT_RS15150 0.85 +4.1 3,448,745 - BPHYT_RS15150 0.85 +0.3 3,448,769 - BPHYT_RS15150 0.86 +1.3 3,448,916 + +3.4 3,449,018 - +1.2 3,449,179 + BPHYT_RS15155 0.11 +2.8 3,449,338 - BPHYT_RS15155 0.34 +1.2 3,449,338 - BPHYT_RS15155 0.34 +1.6 3,449,349 - BPHYT_RS15155 0.36 +1.8 3,449,479 - BPHYT_RS15155 0.55 +2.9 3,449,479 - BPHYT_RS15155 0.55 +2.6 3,449,491 - BPHYT_RS15155 0.57 +1.8 3,449,701 + BPHYT_RS15155 0.87 +3.1 3,449,732 + +3.3 3,449,732 + +2.7 3,449,732 + +2.7 3,449,733 - +0.5 3,449,892 + BPHYT_RS15160 0.27 +3.5 3,449,923 + BPHYT_RS15160 0.34 +2.3 3,449,982 - BPHYT_RS15160 0.48 +0.3 3,449,982 - BPHYT_RS15160 0.48 +2.3 3,449,999 + BPHYT_RS15160 0.52 +2.8 3,450,062 + BPHYT_RS15160 0.66 +2.9
Or see this region's nucleotide sequence