Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13315

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13310 and BPHYT_RS13315 are separated by 68 nucleotidesBPHYT_RS13315 and BPHYT_RS13320 are separated by 135 nucleotides BPHYT_RS13310: BPHYT_RS13310 - dihydropyrimidine dehydrogenase subunit B, at 3,038,812 to 3,040,146 _RS13310 BPHYT_RS13315: BPHYT_RS13315 - dihydropyrimidine dehydrogenase subunit A, at 3,040,215 to 3,041,564 _RS13315 BPHYT_RS13320: BPHYT_RS13320 - allantoate amidohydrolase, at 3,041,700 to 3,042,980 _RS13320 Position (kb) 3040 3041 3042Strain fitness (log2 ratio) -2 -1 0 1at 3039.575 kb on + strand, within BPHYT_RS13310at 3040.061 kb on + strandat 3040.129 kb on - strandat 3040.182 kb on + strandat 3040.228 kb on - strandat 3040.228 kb on - strandat 3040.776 kb on + strand, within BPHYT_RS13315at 3041.330 kb on + strand, within BPHYT_RS13315at 3041.434 kb on + strandat 3041.745 kb on + strandat 3041.746 kb on - strandat 3041.857 kb on + strand, within BPHYT_RS13320

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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3,039,575 + BPHYT_RS13310 0.57 -1.4
3,040,061 + +0.4
3,040,129 - +0.3
3,040,182 + +1.4
3,040,228 - -2.3
3,040,228 - -0.6
3,040,776 + BPHYT_RS13315 0.42 -0.5
3,041,330 + BPHYT_RS13315 0.83 +0.7
3,041,434 + -2.2
3,041,745 + -0.6
3,041,746 - -1.4
3,041,857 + BPHYT_RS13320 0.12 -2.6

Or see this region's nucleotide sequence