Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10255 and BPHYT_RS10260 are separated by 65 nucleotides BPHYT_RS10260 and BPHYT_RS10265 are separated by 28 nucleotides BPHYT_RS10265 and BPHYT_RS10270 are separated by 45 nucleotides
BPHYT_RS10255: BPHYT_RS10255 - LysR family transcriptional regulator, at 2,331,356 to 2,332,252
_RS10255
BPHYT_RS10260: BPHYT_RS10260 - hypothetical protein, at 2,332,318 to 2,332,719
_RS10260
BPHYT_RS10265: BPHYT_RS10265 - aminotransferase, at 2,332,748 to 2,333,872
_RS10265
BPHYT_RS10270: BPHYT_RS10270 - D-amino acid dehydrogenase, at 2,333,918 to 2,335,159
_RS10270
Position (kb)
2332
2333
2334 Strain fitness (log2 ratio)
-1
0
1 at 2332.108 kb on - strand, within BPHYT_RS10255 at 2332.475 kb on - strand, within BPHYT_RS10260 at 2332.972 kb on - strand, within BPHYT_RS10265 at 2333.261 kb on - strand, within BPHYT_RS10265 at 2333.292 kb on + strand, within BPHYT_RS10265 at 2333.811 kb on + strand at 2333.889 kb on - strand at 2333.911 kb on - strand at 2334.273 kb on + strand, within BPHYT_RS10270 at 2334.328 kb on - strand, within BPHYT_RS10270 at 2334.339 kb on + strand, within BPHYT_RS10270
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 2,332,108 - BPHYT_RS10255 0.84 -0.1 2,332,475 - BPHYT_RS10260 0.39 -1.0 2,332,972 - BPHYT_RS10265 0.20 -0.4 2,333,261 - BPHYT_RS10265 0.46 -0.4 2,333,292 + BPHYT_RS10265 0.48 +0.5 2,333,811 + +0.4 2,333,889 - -0.6 2,333,911 - -0.7 2,334,273 + BPHYT_RS10270 0.29 +0.3 2,334,328 - BPHYT_RS10270 0.33 -0.8 2,334,339 + BPHYT_RS10270 0.34 +0.0
Or see this region's nucleotide sequence