Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08060

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08050 and BPHYT_RS08055 are separated by 137 nucleotidesBPHYT_RS08055 and BPHYT_RS08060 are separated by 157 nucleotidesBPHYT_RS08060 and BPHYT_RS08065 overlap by 4 nucleotides BPHYT_RS08050: BPHYT_RS08050 - pseudouridine synthase, at 1,835,368 to 1,837,248 _RS08050 BPHYT_RS08055: BPHYT_RS08055 - hypothetical protein, at 1,837,386 to 1,837,622 _RS08055 BPHYT_RS08060: BPHYT_RS08060 - ribosome maturation factor RimP, at 1,837,780 to 1,838,238 _RS08060 BPHYT_RS08065: BPHYT_RS08065 - transcription termination factor NusA, at 1,838,235 to 1,839,710 _RS08065 Position (kb) 1837 1838 1839Strain fitness (log2 ratio) -1 0 1 2 3at 1837.291 kb on - strandat 1837.370 kb on + strandat 1837.371 kb on - strandat 1837.384 kb on - strandat 1837.768 kb on + strandat 1837.768 kb on + strandat 1837.768 kb on + strandat 1837.869 kb on + strand, within BPHYT_RS08060at 1837.869 kb on + strand, within BPHYT_RS08060at 1837.869 kb on + strand, within BPHYT_RS08060at 1837.869 kb on + strand, within BPHYT_RS08060at 1837.869 kb on + strand, within BPHYT_RS08060at 1837.873 kb on + strand, within BPHYT_RS08060at 1837.873 kb on + strand, within BPHYT_RS08060at 1837.963 kb on + strand, within BPHYT_RS08060

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,837,291 - -0.9
1,837,370 + +0.2
1,837,371 - -0.9
1,837,384 - -0.8
1,837,768 + +0.4
1,837,768 + +0.0
1,837,768 + +0.4
1,837,869 + BPHYT_RS08060 0.19 +2.3
1,837,869 + BPHYT_RS08060 0.19 +1.5
1,837,869 + BPHYT_RS08060 0.19 -0.0
1,837,869 + BPHYT_RS08060 0.19 +0.9
1,837,869 + BPHYT_RS08060 0.19 +2.8
1,837,873 + BPHYT_RS08060 0.20 +0.4
1,837,873 + BPHYT_RS08060 0.20 -0.4
1,837,963 + BPHYT_RS08060 0.40 +0.9

Or see this region's nucleotide sequence