Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04385

Experiment: 24hr PHYTO4 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04380 and BPHYT_RS04385 are separated by 5 nucleotidesBPHYT_RS04385 and BPHYT_RS04390 are separated by 61 nucleotides BPHYT_RS04380: BPHYT_RS04380 - glycosyl transferase, at 1,008,589 to 1,009,620 _RS04380 BPHYT_RS04385: BPHYT_RS04385 - nucleoside-diphosphate sugar oxidoreductase, at 1,009,626 to 1,011,503 _RS04385 BPHYT_RS04390: BPHYT_RS04390 - glycosyl transferase, at 1,011,565 to 1,012,665 _RS04390 Position (kb) 1009 1010 1011 1012Strain fitness (log2 ratio) -1 0 1 2 3at 1009.652 kb on - strandat 1009.711 kb on - strandat 1009.731 kb on + strandat 1009.736 kb on - strandat 1009.952 kb on - strand, within BPHYT_RS04385at 1010.029 kb on - strand, within BPHYT_RS04385at 1010.435 kb on + strand, within BPHYT_RS04385at 1010.587 kb on - strand, within BPHYT_RS04385at 1010.793 kb on + strand, within BPHYT_RS04385at 1011.201 kb on + strand, within BPHYT_RS04385at 1011.473 kb on + strandat 1011.473 kb on + strandat 1011.474 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10
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1,009,652 - +2.7
1,009,711 - +2.7
1,009,731 + +1.1
1,009,736 - +1.6
1,009,952 - BPHYT_RS04385 0.17 +1.9
1,010,029 - BPHYT_RS04385 0.21 +1.1
1,010,435 + BPHYT_RS04385 0.43 +2.7
1,010,587 - BPHYT_RS04385 0.51 +3.5
1,010,793 + BPHYT_RS04385 0.62 +1.4
1,011,201 + BPHYT_RS04385 0.84 +3.1
1,011,473 + -0.3
1,011,473 + +0.9
1,011,474 - +0.1

Or see this region's nucleotide sequence