Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04315 and BPHYT_RS04320 are separated by 120 nucleotides BPHYT_RS04320 and BPHYT_RS04325 are separated by 197 nucleotides
BPHYT_RS04315: BPHYT_RS04315 - UDP-N-acetylglucosamine 2-epimerase, at 993,169 to 994,305
_RS04315
BPHYT_RS04320: BPHYT_RS04320 - hypothetical protein, at 994,426 to 995,727
_RS04320
BPHYT_RS04325: BPHYT_RS04325 - acyltransferase 3, at 995,925 to 997,088
_RS04325
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 993.693 kb on + strand, within BPHYT_RS04315 at 993.713 kb on - strand, within BPHYT_RS04315 at 993.713 kb on - strand, within BPHYT_RS04315 at 993.895 kb on - strand, within BPHYT_RS04315 at 993.912 kb on + strand, within BPHYT_RS04315 at 993.913 kb on - strand, within BPHYT_RS04315 at 993.970 kb on + strand, within BPHYT_RS04315 at 993.971 kb on - strand, within BPHYT_RS04315 at 993.991 kb on + strand, within BPHYT_RS04315 at 994.029 kb on + strand, within BPHYT_RS04315 at 994.030 kb on - strand, within BPHYT_RS04315 at 994.610 kb on + strand, within BPHYT_RS04320 at 994.666 kb on + strand, within BPHYT_RS04320 at 994.675 kb on + strand, within BPHYT_RS04320 at 994.712 kb on - strand, within BPHYT_RS04320 at 994.712 kb on - strand, within BPHYT_RS04320 at 994.811 kb on - strand, within BPHYT_RS04320 at 994.811 kb on - strand, within BPHYT_RS04320 at 995.003 kb on + strand, within BPHYT_RS04320 at 995.003 kb on + strand, within BPHYT_RS04320 at 995.253 kb on + strand, within BPHYT_RS04320 at 995.298 kb on + strand, within BPHYT_RS04320 at 995.299 kb on - strand, within BPHYT_RS04320 at 995.300 kb on + strand, within BPHYT_RS04320 at 995.300 kb on + strand, within BPHYT_RS04320 at 995.301 kb on - strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.477 kb on - strand, within BPHYT_RS04320 at 995.634 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.766 kb on - strand at 995.924 kb on + strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.147 kb on - strand, within BPHYT_RS04325 at 996.150 kb on + strand, within BPHYT_RS04325 at 996.151 kb on - strand, within BPHYT_RS04325 at 996.151 kb on - strand, within BPHYT_RS04325 at 996.179 kb on + strand, within BPHYT_RS04325 at 996.180 kb on - strand, within BPHYT_RS04325 at 996.301 kb on - strand, within BPHYT_RS04325 at 996.363 kb on + strand, within BPHYT_RS04325 at 996.487 kb on + strand, within BPHYT_RS04325 at 996.509 kb on + strand, within BPHYT_RS04325 at 996.540 kb on - strand, within BPHYT_RS04325 at 996.705 kb on + strand, within BPHYT_RS04325 at 996.716 kb on + strand, within BPHYT_RS04325
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 993,693 + BPHYT_RS04315 0.46 +2.5 993,713 - BPHYT_RS04315 0.48 +3.1 993,713 - BPHYT_RS04315 0.48 +3.2 993,895 - BPHYT_RS04315 0.64 +3.3 993,912 + BPHYT_RS04315 0.65 +0.7 993,913 - BPHYT_RS04315 0.65 +1.9 993,970 + BPHYT_RS04315 0.70 +2.8 993,971 - BPHYT_RS04315 0.71 +3.4 993,991 + BPHYT_RS04315 0.72 +2.2 994,029 + BPHYT_RS04315 0.76 +2.3 994,030 - BPHYT_RS04315 0.76 +3.9 994,610 + BPHYT_RS04320 0.14 -0.4 994,666 + BPHYT_RS04320 0.18 -1.6 994,675 + BPHYT_RS04320 0.19 +0.4 994,712 - BPHYT_RS04320 0.22 -0.4 994,712 - BPHYT_RS04320 0.22 -0.5 994,811 - BPHYT_RS04320 0.30 -1.4 994,811 - BPHYT_RS04320 0.30 -0.3 995,003 + BPHYT_RS04320 0.44 -0.7 995,003 + BPHYT_RS04320 0.44 -0.7 995,253 + BPHYT_RS04320 0.64 +0.2 995,298 + BPHYT_RS04320 0.67 -1.5 995,299 - BPHYT_RS04320 0.67 -0.3 995,300 + BPHYT_RS04320 0.67 +0.3 995,300 + BPHYT_RS04320 0.67 +1.7 995,301 - BPHYT_RS04320 0.67 +0.7 995,344 + BPHYT_RS04320 0.71 -1.4 995,344 + BPHYT_RS04320 0.71 -0.0 995,344 + BPHYT_RS04320 0.71 -2.3 995,477 - BPHYT_RS04320 0.81 -1.0 995,634 + -1.0 995,765 + +0.1 995,765 + -0.3 995,765 + -0.1 995,765 + -0.6 995,766 - +0.3 995,924 + -1.0 996,034 - -0.1 996,034 - +0.2 996,034 - -1.0 996,034 - +2.7 996,147 - BPHYT_RS04325 0.19 +0.8 996,150 + BPHYT_RS04325 0.19 -0.1 996,151 - BPHYT_RS04325 0.19 -0.9 996,151 - BPHYT_RS04325 0.19 +0.2 996,179 + BPHYT_RS04325 0.22 +0.4 996,180 - BPHYT_RS04325 0.22 -0.8 996,301 - BPHYT_RS04325 0.32 +1.1 996,363 + BPHYT_RS04325 0.38 -0.7 996,487 + BPHYT_RS04325 0.48 -0.8 996,509 + BPHYT_RS04325 0.50 -0.5 996,540 - BPHYT_RS04325 0.53 -0.7 996,705 + BPHYT_RS04325 0.67 -0.7 996,716 + BPHYT_RS04325 0.68 +0.5
Or see this region's nucleotide sequence