Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04255 and BPHYT_RS04260 are separated by 193 nucleotides BPHYT_RS04260 and BPHYT_RS04265 are separated by 469 nucleotides
BPHYT_RS04255: BPHYT_RS04255 - diadenosine tetraphosphatase, at 974,908 to 975,786
_RS04255
BPHYT_RS04260: BPHYT_RS04260 - GDP-6-deoxy-D-lyxo-4-hexulose reductase, at 975,980 to 976,891
_RS04260
BPHYT_RS04265: BPHYT_RS04265 - dTDP-glucose 4,6-dehydratase, at 977,361 to 978,422
_RS04265
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 975.477 kb on + strand, within BPHYT_RS04255 at 975.771 kb on + strand at 975.772 kb on - strand at 975.772 kb on - strand at 975.772 kb on - strand at 975.809 kb on + strand at 975.809 kb on + strand at 975.857 kb on + strand at 975.867 kb on + strand at 975.867 kb on + strand at 975.867 kb on + strand at 975.868 kb on - strand at 975.868 kb on - strand at 975.868 kb on - strand at 976.006 kb on + strand at 976.013 kb on - strand at 976.196 kb on + strand, within BPHYT_RS04260 at 976.197 kb on - strand, within BPHYT_RS04260 at 976.228 kb on - strand, within BPHYT_RS04260 at 976.353 kb on - strand, within BPHYT_RS04260 at 976.496 kb on + strand, within BPHYT_RS04260 at 976.497 kb on - strand, within BPHYT_RS04260 at 976.498 kb on + strand, within BPHYT_RS04260 at 976.499 kb on - strand, within BPHYT_RS04260 at 976.619 kb on + strand, within BPHYT_RS04260 at 976.634 kb on + strand, within BPHYT_RS04260 at 976.634 kb on + strand, within BPHYT_RS04260 at 976.635 kb on - strand, within BPHYT_RS04260 at 976.864 kb on + strand at 976.889 kb on + strand at 977.048 kb on + strand at 977.187 kb on + strand at 977.187 kb on + strand at 977.209 kb on + strand at 977.225 kb on + strand at 977.225 kb on + strand at 977.225 kb on + strand at 977.226 kb on - strand at 977.347 kb on + strand at 977.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 975,477 + BPHYT_RS04255 0.65 -0.2 975,771 + -0.6 975,772 - -0.6 975,772 - +2.4 975,772 - -2.2 975,809 + +0.6 975,809 + +0.7 975,857 + -1.9 975,867 + +0.1 975,867 + -1.1 975,867 + -1.9 975,868 - -1.8 975,868 - -0.5 975,868 - -0.8 976,006 + -0.3 976,013 - -1.1 976,196 + BPHYT_RS04260 0.24 +0.5 976,197 - BPHYT_RS04260 0.24 -0.5 976,228 - BPHYT_RS04260 0.27 +0.4 976,353 - BPHYT_RS04260 0.41 -1.1 976,496 + BPHYT_RS04260 0.57 +0.0 976,497 - BPHYT_RS04260 0.57 +0.8 976,498 + BPHYT_RS04260 0.57 +0.2 976,499 - BPHYT_RS04260 0.57 -0.3 976,619 + BPHYT_RS04260 0.70 -0.4 976,634 + BPHYT_RS04260 0.72 +0.5 976,634 + BPHYT_RS04260 0.72 +0.4 976,635 - BPHYT_RS04260 0.72 -0.6 976,864 + -0.2 976,889 + +1.7 977,048 + -1.4 977,187 + -0.5 977,187 + -2.0 977,209 + -3.5 977,225 + -1.2 977,225 + -1.4 977,225 + -1.1 977,226 - +0.1 977,347 + +0.7 977,347 + -0.6
Or see this region's nucleotide sequence