Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03730 and BPHYT_RS03735 are separated by 198 nucleotides BPHYT_RS03735 and BPHYT_RS03740 overlap by 4 nucleotides BPHYT_RS03740 and BPHYT_RS03745 overlap by 4 nucleotides BPHYT_RS03745 and BPHYT_RS03750 are separated by 137 nucleotides
BPHYT_RS03730: BPHYT_RS03730 - transcriptional repressor NrdR, at 846,523 to 847,005
_RS03730
BPHYT_RS03735: BPHYT_RS03735 - pilus assembly protein, at 847,204 to 847,770
_RS03735
BPHYT_RS03740: BPHYT_RS03740 - hypothetical protein, at 847,767 to 848,213
_RS03740
BPHYT_RS03745: BPHYT_RS03745 - cleavage protein, at 848,210 to 849,022
_RS03745
BPHYT_RS03750: BPHYT_RS03750 - hypothetical protein, at 849,160 to 849,639
_RS03750
Position (kb)
847
848
849 Strain fitness (log2 ratio)
-2
-1
0
1 at 846.800 kb on - strand, within BPHYT_RS03730 at 846.893 kb on - strand, within BPHYT_RS03730 at 847.385 kb on - strand at 847.385 kb on - strand, within BPHYT_RS03735 at 847.385 kb on - strand, within BPHYT_RS03735 at 847.579 kb on - strand, within BPHYT_RS03735 at 847.635 kb on + strand, within BPHYT_RS03735 at 847.776 kb on + strand at 847.776 kb on + strand at 847.777 kb on - strand at 847.792 kb on - strand at 848.068 kb on + strand, within BPHYT_RS03740 at 848.068 kb on + strand, within BPHYT_RS03740 at 848.191 kb on + strand at 848.191 kb on + strand at 848.283 kb on + strand at 848.309 kb on + strand, within BPHYT_RS03745 at 848.552 kb on + strand, within BPHYT_RS03745 at 848.675 kb on + strand, within BPHYT_RS03745 at 848.718 kb on - strand, within BPHYT_RS03745 at 848.790 kb on - strand, within BPHYT_RS03745 at 848.883 kb on + strand, within BPHYT_RS03745 at 848.884 kb on - strand, within BPHYT_RS03745 at 848.919 kb on - strand at 848.919 kb on - strand, within BPHYT_RS03745 at 849.018 kb on + strand at 849.137 kb on + strand at 849.138 kb on - strand at 849.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 846,800 - BPHYT_RS03730 0.57 +1.2 846,893 - BPHYT_RS03730 0.77 +0.1 847,385 - +1.2 847,385 - BPHYT_RS03735 0.32 -0.2 847,385 - BPHYT_RS03735 0.32 -0.7 847,579 - BPHYT_RS03735 0.66 +1.5 847,635 + BPHYT_RS03735 0.76 -0.1 847,776 + -1.3 847,776 + -1.3 847,777 - -0.1 847,792 - +0.4 848,068 + BPHYT_RS03740 0.67 -1.7 848,068 + BPHYT_RS03740 0.67 -1.3 848,191 + -1.5 848,191 + -1.7 848,283 + -0.1 848,309 + BPHYT_RS03745 0.12 -0.3 848,552 + BPHYT_RS03745 0.42 -0.8 848,675 + BPHYT_RS03745 0.57 -1.6 848,718 - BPHYT_RS03745 0.62 -0.9 848,790 - BPHYT_RS03745 0.71 -0.1 848,883 + BPHYT_RS03745 0.83 +0.1 848,884 - BPHYT_RS03745 0.83 -0.4 848,919 - -0.3 848,919 - BPHYT_RS03745 0.87 +0.3 849,018 + -0.8 849,137 + +0.3 849,138 - -1.8 849,138 - +0.5
Or see this region's nucleotide sequence