Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01695 and BPHYT_RS01700 are separated by 65 nucleotides BPHYT_RS01700 and BPHYT_RS01705 are separated by 272 nucleotides BPHYT_RS01705 and BPHYT_RS01710 are separated by 241 nucleotides
BPHYT_RS01695: BPHYT_RS01695 - L-glyceraldehyde 3-phosphate reductase, at 383,542 to 384,585
_RS01695
BPHYT_RS01700: BPHYT_RS01700 - membrane protein, at 384,651 to 384,836
_RS01700
BPHYT_RS01705: BPHYT_RS01705 - MFS transporter, at 385,109 to 386,311
_RS01705
BPHYT_RS01710: BPHYT_RS01710 - RND transporter, at 386,553 to 388,082
_RS01710
Position (kb) 385
386
387 Strain fitness (log2 ratio) -2
-1
0
1 at 384.130 kb on + strand, within BPHYT_RS01695 at 384.241 kb on + strand, within BPHYT_RS01695 at 384.242 kb on - strand, within BPHYT_RS01695 at 384.826 kb on + strand at 384.826 kb on + strand at 384.848 kb on + strand at 384.848 kb on + strand at 384.849 kb on - strand at 384.849 kb on - strand at 384.879 kb on + strand at 384.880 kb on - strand at 384.914 kb on - strand at 384.915 kb on + strand at 384.916 kb on - strand at 384.916 kb on - strand at 384.936 kb on - strand at 384.936 kb on - strand at 384.937 kb on + strand at 385.053 kb on + strand at 385.054 kb on - strand at 385.231 kb on - strand, within BPHYT_RS01705 at 385.257 kb on + strand, within BPHYT_RS01705 at 385.258 kb on - strand, within BPHYT_RS01705 at 385.260 kb on + strand, within BPHYT_RS01705 at 385.272 kb on + strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.596 kb on + strand, within BPHYT_RS01705 at 385.597 kb on - strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.930 kb on - strand, within BPHYT_RS01705 at 386.048 kb on - strand, within BPHYT_RS01705 at 386.048 kb on - strand, within BPHYT_RS01705 at 386.215 kb on - strand at 386.356 kb on + strand at 386.357 kb on - strand at 386.448 kb on + strand at 386.448 kb on + strand at 386.555 kb on - strand at 386.769 kb on - strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.044 kb on - strand, within BPHYT_RS01710 at 387.044 kb on - strand, within BPHYT_RS01710
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 384,130 + BPHYT_RS01695 0.56 -1.4 384,241 + BPHYT_RS01695 0.67 -0.3 384,242 - BPHYT_RS01695 0.67 -1.2 384,826 + -0.1 384,826 + +0.3 384,848 + +0.5 384,848 + -0.7 384,849 - -0.8 384,849 - -1.6 384,879 + -1.4 384,880 - +0.5 384,914 - +0.4 384,915 + -0.8 384,916 - +0.5 384,916 - +0.1 384,936 - +0.8 384,936 - +0.4 384,937 + -0.5 385,053 + -0.8 385,054 - -2.4 385,231 - BPHYT_RS01705 0.10 -1.9 385,257 + BPHYT_RS01705 0.12 -0.7 385,258 - BPHYT_RS01705 0.12 -1.4 385,260 + BPHYT_RS01705 0.13 +0.3 385,272 + BPHYT_RS01705 0.14 -1.2 385,519 - BPHYT_RS01705 0.34 +0.5 385,519 - BPHYT_RS01705 0.34 -1.1 385,519 - BPHYT_RS01705 0.34 +0.9 385,519 - BPHYT_RS01705 0.34 +0.7 385,560 + BPHYT_RS01705 0.37 +1.1 385,560 + BPHYT_RS01705 0.37 -0.6 385,561 - BPHYT_RS01705 0.38 +0.0 385,561 - BPHYT_RS01705 0.38 -0.6 385,590 + BPHYT_RS01705 0.40 +0.4 385,590 + BPHYT_RS01705 0.40 +0.1 385,590 + BPHYT_RS01705 0.40 -1.2 385,596 + BPHYT_RS01705 0.40 -1.6 385,597 - BPHYT_RS01705 0.41 -0.3 385,929 + BPHYT_RS01705 0.68 +0.2 385,929 + BPHYT_RS01705 0.68 +0.0 385,929 + BPHYT_RS01705 0.68 +0.2 385,930 - BPHYT_RS01705 0.68 -0.7 386,048 - BPHYT_RS01705 0.78 -2.5 386,048 - BPHYT_RS01705 0.78 -0.5 386,215 - -0.8 386,356 + +0.2 386,357 - +0.3 386,448 + -0.8 386,448 + -0.5 386,555 - -0.4 386,769 - BPHYT_RS01710 0.14 -1.1 387,043 + BPHYT_RS01710 0.32 -0.9 387,043 + BPHYT_RS01710 0.32 +1.1 387,043 + BPHYT_RS01710 0.32 +0.5 387,043 + BPHYT_RS01710 0.32 +0.3 387,044 - BPHYT_RS01710 0.32 -0.6 387,044 - BPHYT_RS01710 0.32 +0.0
Or see this region's nucleotide sequence