Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01450 and BPHYT_RS01455 are separated by 244 nucleotides BPHYT_RS01455 and BPHYT_RS01460 are separated by 31 nucleotides BPHYT_RS01460 and BPHYT_RS01465 are separated by 114 nucleotides BPHYT_RS01465 and BPHYT_RS01470 are separated by 103 nucleotides
BPHYT_RS01450: BPHYT_RS01450 - DNA-binding protein, at 331,693 to 332,961
_RS01450
BPHYT_RS01455: BPHYT_RS01455 - peptide deformylase, at 333,206 to 333,709
_RS01455
BPHYT_RS01460: BPHYT_RS01460 - methionyl-tRNA formyltransferase, at 333,741 to 334,727
_RS01460
BPHYT_RS01465: BPHYT_RS01465 - lysine transporter LysE, at 334,842 to 335,486
_RS01465
BPHYT_RS01470: BPHYT_RS01470 - protease HtpX homolog, at 335,590 to 336,447
_RS01470
Position (kb) 333
334
335 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 332.807 kb on - strand, within BPHYT_RS01450 at 332.979 kb on - strand at 332.979 kb on - strand at 332.979 kb on - strand at 332.979 kb on - strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.213 kb on + strand at 333.644 kb on + strand, within BPHYT_RS01455 at 333.707 kb on + strand at 333.707 kb on + strand at 333.707 kb on + strand at 333.707 kb on + strand at 334.725 kb on + strand at 334.725 kb on + strand at 334.726 kb on - strand at 334.802 kb on + strand at 334.813 kb on - strand at 334.920 kb on + strand, within BPHYT_RS01465 at 334.920 kb on + strand, within BPHYT_RS01465 at 334.920 kb on + strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.921 kb on - strand, within BPHYT_RS01465 at 334.922 kb on + strand, within BPHYT_RS01465 at 334.922 kb on + strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 334.923 kb on - strand, within BPHYT_RS01465 at 335.060 kb on + strand, within BPHYT_RS01465 at 335.060 kb on + strand, within BPHYT_RS01465 at 335.084 kb on + strand, within BPHYT_RS01465 at 335.085 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.089 kb on - strand, within BPHYT_RS01465 at 335.098 kb on - strand, within BPHYT_RS01465 at 335.098 kb on - strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.236 kb on + strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.237 kb on - strand, within BPHYT_RS01465 at 335.513 kb on + strand at 335.514 kb on - strand at 335.520 kb on + strand at 335.520 kb on + strand at 335.520 kb on + strand at 335.525 kb on + strand at 335.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 332,807 - BPHYT_RS01450 0.88 -1.8 332,979 - +1.2 332,979 - +0.1 332,979 - +2.1 332,979 - -1.1 333,111 + -0.6 333,111 + +0.7 333,111 + +0.3 333,111 + -1.5 333,112 - +0.0 333,112 - -1.7 333,112 - -1.2 333,112 - -0.4 333,112 - -1.7 333,112 - -1.3 333,112 - +0.1 333,112 - -0.5 333,112 - -1.5 333,213 + +5.2 333,644 + BPHYT_RS01455 0.87 +3.5 333,707 + -0.7 333,707 + -0.4 333,707 + +2.8 333,707 + +1.5 334,725 + +1.2 334,725 + -1.1 334,726 - -0.9 334,802 + -0.7 334,813 - -0.4 334,920 + BPHYT_RS01465 0.12 +2.0 334,920 + BPHYT_RS01465 0.12 -2.6 334,920 + BPHYT_RS01465 0.12 +0.7 334,921 - BPHYT_RS01465 0.12 -0.6 334,921 - BPHYT_RS01465 0.12 +0.4 334,921 - BPHYT_RS01465 0.12 +0.1 334,921 - BPHYT_RS01465 0.12 -1.2 334,921 - BPHYT_RS01465 0.12 +1.0 334,921 - BPHYT_RS01465 0.12 -2.3 334,922 + BPHYT_RS01465 0.12 -0.2 334,922 + BPHYT_RS01465 0.12 -0.6 334,923 - BPHYT_RS01465 0.13 +0.7 334,923 - BPHYT_RS01465 0.13 -1.2 334,923 - BPHYT_RS01465 0.13 +0.0 334,923 - BPHYT_RS01465 0.13 +0.7 334,923 - BPHYT_RS01465 0.13 -0.9 334,923 - BPHYT_RS01465 0.13 -0.3 335,060 + BPHYT_RS01465 0.34 -1.4 335,060 + BPHYT_RS01465 0.34 -0.5 335,084 + BPHYT_RS01465 0.38 -1.5 335,085 - BPHYT_RS01465 0.38 +2.8 335,089 - BPHYT_RS01465 0.38 +0.7 335,089 - BPHYT_RS01465 0.38 -0.5 335,089 - BPHYT_RS01465 0.38 -1.3 335,098 - BPHYT_RS01465 0.40 -1.4 335,098 - BPHYT_RS01465 0.40 +0.3 335,236 + BPHYT_RS01465 0.61 -1.7 335,236 + BPHYT_RS01465 0.61 -0.0 335,236 + BPHYT_RS01465 0.61 -0.2 335,236 + BPHYT_RS01465 0.61 -2.1 335,236 + BPHYT_RS01465 0.61 -0.8 335,236 + BPHYT_RS01465 0.61 -0.1 335,236 + BPHYT_RS01465 0.61 +1.0 335,236 + BPHYT_RS01465 0.61 -0.9 335,237 - BPHYT_RS01465 0.61 -0.4 335,237 - BPHYT_RS01465 0.61 +0.3 335,237 - BPHYT_RS01465 0.61 +0.1 335,513 + -1.3 335,514 - +0.8 335,520 + -1.1 335,520 + -0.7 335,520 + -0.0 335,525 + +0.1 335,526 - +1.2
Or see this region's nucleotide sequence