Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00820 and BPHYT_RS00825 overlap by 4 nucleotides BPHYT_RS00825 and BPHYT_RS00830 are separated by 45 nucleotides
BPHYT_RS00820: BPHYT_RS00820 - type IV secretory pathway protein VirD4, at 184,673 to 186,457
_RS00820
BPHYT_RS00825: BPHYT_RS00825 - DNA replication protein, at 186,454 to 188,367
_RS00825
BPHYT_RS00830: BPHYT_RS00830 - DNA primase, at 188,413 to 190,002
_RS00830
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.697 kb on - strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.783 kb on + strand, within BPHYT_RS00820 at 185.784 kb on - strand, within BPHYT_RS00820 at 186.227 kb on + strand, within BPHYT_RS00820 at 186.466 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.907 kb on + strand, within BPHYT_RS00825 at 186.908 kb on - strand, within BPHYT_RS00825 at 187.457 kb on + strand, within BPHYT_RS00825 at 187.491 kb on - strand, within BPHYT_RS00825 at 187.580 kb on + strand, within BPHYT_RS00825 at 187.580 kb on + strand at 187.580 kb on + strand, within BPHYT_RS00825 at 187.580 kb on + strand, within BPHYT_RS00825 at 187.581 kb on - strand, within BPHYT_RS00825 at 187.812 kb on + strand, within BPHYT_RS00825 at 187.812 kb on + strand, within BPHYT_RS00825 at 187.812 kb on + strand, within BPHYT_RS00825 at 187.813 kb on - strand, within BPHYT_RS00825 at 187.813 kb on - strand, within BPHYT_RS00825 at 187.996 kb on + strand, within BPHYT_RS00825 at 188.267 kb on + strand at 188.268 kb on - strand at 188.764 kb on + strand, within BPHYT_RS00830 at 188.785 kb on + strand, within BPHYT_RS00830 at 188.836 kb on + strand, within BPHYT_RS00830 at 188.913 kb on + strand, within BPHYT_RS00830 at 188.992 kb on + strand, within BPHYT_RS00830 at 188.993 kb on - strand, within BPHYT_RS00830 at 189.093 kb on - strand, within BPHYT_RS00830 at 189.308 kb on - strand, within BPHYT_RS00830
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 185,630 + BPHYT_RS00820 0.54 +1.1 185,630 + BPHYT_RS00820 0.54 -0.2 185,642 + BPHYT_RS00820 0.54 +0.2 185,642 + BPHYT_RS00820 0.54 -0.1 185,697 - BPHYT_RS00820 0.57 -0.2 185,750 + BPHYT_RS00820 0.60 +0.2 185,750 + BPHYT_RS00820 0.60 -0.5 185,750 + BPHYT_RS00820 0.60 +0.2 185,750 + BPHYT_RS00820 0.60 -2.2 185,751 - BPHYT_RS00820 0.60 -0.0 185,751 - BPHYT_RS00820 0.60 +0.1 185,751 - BPHYT_RS00820 0.60 -0.8 185,783 + BPHYT_RS00820 0.62 -1.0 185,784 - BPHYT_RS00820 0.62 +0.2 186,227 + BPHYT_RS00820 0.87 -2.0 186,466 + -1.1 186,619 + -0.4 186,619 + -0.0 186,619 + -0.3 186,619 + -1.0 186,907 + BPHYT_RS00825 0.24 -0.0 186,908 - BPHYT_RS00825 0.24 -0.1 187,457 + BPHYT_RS00825 0.52 +1.2 187,491 - BPHYT_RS00825 0.54 +0.2 187,580 + BPHYT_RS00825 0.59 -0.6 187,580 + +2.2 187,580 + BPHYT_RS00825 0.59 +2.5 187,580 + BPHYT_RS00825 0.59 -0.2 187,581 - BPHYT_RS00825 0.59 -0.1 187,812 + BPHYT_RS00825 0.71 -0.8 187,812 + BPHYT_RS00825 0.71 -0.4 187,812 + BPHYT_RS00825 0.71 -0.0 187,813 - BPHYT_RS00825 0.71 -1.4 187,813 - BPHYT_RS00825 0.71 -0.3 187,996 + BPHYT_RS00825 0.81 +0.4 188,267 + +0.2 188,268 - -0.1 188,764 + BPHYT_RS00830 0.22 +0.1 188,785 + BPHYT_RS00830 0.23 +1.0 188,836 + BPHYT_RS00830 0.27 +1.0 188,913 + BPHYT_RS00830 0.31 -0.3 188,992 + BPHYT_RS00830 0.36 +0.2 188,993 - BPHYT_RS00830 0.36 +1.3 189,093 - BPHYT_RS00830 0.43 +0.3 189,308 - BPHYT_RS00830 0.56 +0.4
Or see this region's nucleotide sequence