Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00335 and BPHYT_RS00340 are separated by 16 nucleotides BPHYT_RS00340 and BPHYT_RS00345 are separated by 223 nucleotides
BPHYT_RS00335: BPHYT_RS00335 - peptidase M19, at 84,101 to 85,099
_RS00335
BPHYT_RS00340: BPHYT_RS00340 - hypothetical protein, at 85,116 to 85,709
_RS00340
BPHYT_RS00345: BPHYT_RS00345 - MFS transporter, at 85,933 to 87,156
_RS00345
Position (kb) 85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 84.232 kb on + strand, within BPHYT_RS00335 at 84.233 kb on - strand, within BPHYT_RS00335 at 84.237 kb on - strand, within BPHYT_RS00335 at 84.361 kb on + strand, within BPHYT_RS00335 at 84.458 kb on + strand, within BPHYT_RS00335 at 84.491 kb on + strand, within BPHYT_RS00335 at 84.491 kb on + strand, within BPHYT_RS00335 at 84.956 kb on - strand, within BPHYT_RS00335 at 84.986 kb on - strand, within BPHYT_RS00335 at 85.134 kb on - strand at 85.134 kb on - strand at 85.230 kb on - strand, within BPHYT_RS00340 at 85.466 kb on - strand, within BPHYT_RS00340 at 85.603 kb on - strand, within BPHYT_RS00340 at 85.777 kb on + strand at 85.777 kb on + strand at 85.778 kb on - strand at 85.786 kb on - strand at 85.927 kb on - strand at 85.934 kb on + strand at 85.951 kb on - strand at 85.951 kb on - strand at 86.086 kb on + strand, within BPHYT_RS00345 at 86.087 kb on - strand, within BPHYT_RS00345 at 86.110 kb on - strand, within BPHYT_RS00345 at 86.238 kb on + strand, within BPHYT_RS00345 at 86.239 kb on - strand, within BPHYT_RS00345 at 86.246 kb on + strand, within BPHYT_RS00345 at 86.259 kb on + strand, within BPHYT_RS00345 at 86.260 kb on - strand, within BPHYT_RS00345 at 86.332 kb on + strand, within BPHYT_RS00345 at 86.383 kb on + strand, within BPHYT_RS00345 at 86.443 kb on + strand, within BPHYT_RS00345 at 86.527 kb on + strand, within BPHYT_RS00345 at 86.527 kb on + strand, within BPHYT_RS00345 at 86.672 kb on - strand, within BPHYT_RS00345 at 86.672 kb on - strand, within BPHYT_RS00345 at 86.698 kb on + strand, within BPHYT_RS00345 at 86.698 kb on + strand, within BPHYT_RS00345
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 84,232 + BPHYT_RS00335 0.13 -0.3 84,233 - BPHYT_RS00335 0.13 -0.5 84,237 - BPHYT_RS00335 0.14 +1.2 84,361 + BPHYT_RS00335 0.26 -1.5 84,458 + BPHYT_RS00335 0.36 -0.0 84,491 + BPHYT_RS00335 0.39 +0.2 84,491 + BPHYT_RS00335 0.39 +0.5 84,956 - BPHYT_RS00335 0.86 +0.8 84,986 - BPHYT_RS00335 0.89 +2.8 85,134 - -0.2 85,134 - -1.6 85,230 - BPHYT_RS00340 0.19 -1.0 85,466 - BPHYT_RS00340 0.59 +0.9 85,603 - BPHYT_RS00340 0.82 +0.7 85,777 + +2.4 85,777 + +0.9 85,778 - +0.9 85,786 - +1.1 85,927 - -0.0 85,934 + -1.3 85,951 - -2.8 85,951 - -0.6 86,086 + BPHYT_RS00345 0.12 +2.8 86,087 - BPHYT_RS00345 0.13 -0.5 86,110 - BPHYT_RS00345 0.14 -0.2 86,238 + BPHYT_RS00345 0.25 -0.2 86,239 - BPHYT_RS00345 0.25 -0.8 86,246 + BPHYT_RS00345 0.26 -0.2 86,259 + BPHYT_RS00345 0.27 +0.6 86,260 - BPHYT_RS00345 0.27 -1.4 86,332 + BPHYT_RS00345 0.33 -1.0 86,383 + BPHYT_RS00345 0.37 -1.3 86,443 + BPHYT_RS00345 0.42 +0.3 86,527 + BPHYT_RS00345 0.49 -0.5 86,527 + BPHYT_RS00345 0.49 -2.4 86,672 - BPHYT_RS00345 0.60 -0.3 86,672 - BPHYT_RS00345 0.60 -0.5 86,698 + BPHYT_RS00345 0.62 -1.2 86,698 + BPHYT_RS00345 0.62 -0.0
Or see this region's nucleotide sequence