Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31065

Experiment: 24hr PHYTO3 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31060 and BPHYT_RS31065 are separated by 7 nucleotidesBPHYT_RS31065 and BPHYT_RS31070 are separated by 4 nucleotidesBPHYT_RS31070 and BPHYT_RS31075 are separated by 19 nucleotides BPHYT_RS31060: BPHYT_RS31060 - capsular polysaccharide biosynthesis protein, at 2,523,542 to 2,524,822 _RS31060 BPHYT_RS31065: BPHYT_RS31065 - polysaccharide biosynthesis protein, at 2,524,830 to 2,526,194 _RS31065 BPHYT_RS31070: BPHYT_RS31070 - acetyltransferase, at 2,526,199 to 2,526,840 _RS31070 BPHYT_RS31075: BPHYT_RS31075 - hypothetical protein, at 2,526,860 to 2,527,996 _RS31075 Position (kb) 2524 2525 2526 2527Strain fitness (log2 ratio) -1 0 1 2at 2524.097 kb on - strand, within BPHYT_RS31060at 2524.230 kb on - strand, within BPHYT_RS31060at 2525.391 kb on + strand, within BPHYT_RS31065at 2525.394 kb on - strand, within BPHYT_RS31065at 2525.395 kb on - strand, within BPHYT_RS31065at 2525.684 kb on - strand, within BPHYT_RS31065at 2527.056 kb on - strand, within BPHYT_RS31075

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1
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2,524,097 - BPHYT_RS31060 0.43 -0.5
2,524,230 - BPHYT_RS31060 0.54 +1.5
2,525,391 + BPHYT_RS31065 0.41 +2.5
2,525,394 - BPHYT_RS31065 0.41 +0.7
2,525,395 - BPHYT_RS31065 0.41 +0.9
2,525,684 - BPHYT_RS31065 0.63 -0.3
2,527,056 - BPHYT_RS31075 0.17 -1.3

Or see this region's nucleotide sequence