Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27890 and BPHYT_RS27895 are separated by 42 nucleotides BPHYT_RS27895 and BPHYT_RS27900 are separated by 172 nucleotides BPHYT_RS27900 and BPHYT_RS27905 are separated by 414 nucleotides
BPHYT_RS27890: BPHYT_RS27890 - hypothetical protein, at 1,822,252 to 1,822,512
_RS27890
BPHYT_RS27895: BPHYT_RS27895 - enoyl-CoA hydratase, at 1,822,555 to 1,822,998
_RS27895
BPHYT_RS27900: BPHYT_RS27900 - 2-hydroxyacid dehydrogenase, at 1,823,171 to 1,824,160
_RS27900
BPHYT_RS27905: BPHYT_RS27905 - MFS transporter, at 1,824,575 to 1,825,882
_RS27905
Position (kb) 1823
1824
1825 Strain fitness (log2 ratio) -1
0
1
2
3 at 1822.417 kb on + strand, within BPHYT_RS27890 at 1822.533 kb on + strand at 1822.589 kb on - strand at 1822.643 kb on + strand, within BPHYT_RS27895 at 1822.853 kb on + strand, within BPHYT_RS27895 at 1822.854 kb on - strand, within BPHYT_RS27895 at 1823.004 kb on + strand at 1823.737 kb on - strand, within BPHYT_RS27900 at 1824.187 kb on + strand at 1824.225 kb on - strand at 1824.308 kb on - strand at 1824.548 kb on - strand at 1825.058 kb on + strand, within BPHYT_RS27905
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 1,822,417 + BPHYT_RS27890 0.63 +2.8 1,822,533 + +0.7 1,822,589 - +0.1 1,822,643 + BPHYT_RS27895 0.20 -1.2 1,822,853 + BPHYT_RS27895 0.67 -0.6 1,822,854 - BPHYT_RS27895 0.67 -0.2 1,823,004 + -0.9 1,823,737 - BPHYT_RS27900 0.57 +0.5 1,824,187 + -0.7 1,824,225 - +0.2 1,824,308 - -0.9 1,824,548 - -1.3 1,825,058 + BPHYT_RS27905 0.37 +0.1
Or see this region's nucleotide sequence