Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27440 and BPHYT_RS27445 are separated by 410 nucleotides BPHYT_RS27445 and BPHYT_RS27450 are separated by 120 nucleotides BPHYT_RS27450 and BPHYT_RS27455 are separated by 292 nucleotides
BPHYT_RS27440: BPHYT_RS27440 - type II citrate synthase, at 1,732,162 to 1,733,460
_RS27440
BPHYT_RS27445: BPHYT_RS27445 - hypothetical protein, at 1,733,871 to 1,734,272
_RS27445
BPHYT_RS27450: BPHYT_RS27450 - alpha/beta hydrolase, at 1,734,393 to 1,735,196
_RS27450
BPHYT_RS27455: BPHYT_RS27455 - rod shape-determining protein MreB, at 1,735,489 to 1,736,541
_RS27455
Position (kb) 1734
1735
1736 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1733.462 kb on + strand at 1734.006 kb on - strand, within BPHYT_RS27445 at 1734.121 kb on - strand, within BPHYT_RS27445 at 1734.271 kb on - strand at 1734.271 kb on - strand at 1734.543 kb on - strand, within BPHYT_RS27450 at 1734.622 kb on + strand, within BPHYT_RS27450 at 1734.623 kb on - strand, within BPHYT_RS27450 at 1735.189 kb on + strand at 1735.267 kb on - strand at 1735.380 kb on - strand at 1735.824 kb on + strand, within BPHYT_RS27455 at 1735.825 kb on - strand, within BPHYT_RS27455
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 1,733,462 + +0.4 1,734,006 - BPHYT_RS27445 0.34 +0.5 1,734,121 - BPHYT_RS27445 0.62 +0.0 1,734,271 - -1.5 1,734,271 - +1.9 1,734,543 - BPHYT_RS27450 0.19 -2.9 1,734,622 + BPHYT_RS27450 0.28 -0.4 1,734,623 - BPHYT_RS27450 0.29 -0.9 1,735,189 + +0.5 1,735,267 - -1.1 1,735,380 - +0.2 1,735,824 + BPHYT_RS27455 0.32 +0.9 1,735,825 - BPHYT_RS27455 0.32 -2.2
Or see this region's nucleotide sequence