Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25890

Experiment: 24hr PHYTO3 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25880 and BPHYT_RS25885 are separated by 32 nucleotidesBPHYT_RS25885 and BPHYT_RS25890 overlap by 1 nucleotidesBPHYT_RS25890 and BPHYT_RS25895 overlap by 4 nucleotidesBPHYT_RS25895 and BPHYT_RS25900 are separated by 38 nucleotides BPHYT_RS25880: BPHYT_RS25880 - type III secretion protein, at 1,387,014 to 1,387,754 _RS25880 BPHYT_RS25885: BPHYT_RS25885 - type III secretion protein, at 1,387,787 to 1,388,476 _RS25885 BPHYT_RS25890: BPHYT_RS25890 - type III secretion protein, at 1,388,476 to 1,388,751 _RS25890 BPHYT_RS25895: BPHYT_RS25895 - type III secretion system protein, at 1,388,748 to 1,389,395 _RS25895 BPHYT_RS25900: BPHYT_RS25900 - hypothetical protein, at 1,389,434 to 1,390,375 _RS25900 Position (kb) 1388 1389Strain fitness (log2 ratio) -1 0 1at 1387.760 kb on + strandat 1387.760 kb on + strandat 1388.173 kb on - strand, within BPHYT_RS25885at 1388.463 kb on - strandat 1389.112 kb on - strand, within BPHYT_RS25895at 1389.703 kb on + strand, within BPHYT_RS25900

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1
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1,387,760 + -0.8
1,387,760 + -1.4
1,388,173 - BPHYT_RS25885 0.56 +0.8
1,388,463 - +0.4
1,389,112 - BPHYT_RS25895 0.56 +0.5
1,389,703 + BPHYT_RS25900 0.29 -0.2

Or see this region's nucleotide sequence