Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24250

Experiment: 24hr PHYTO3 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24245 and BPHYT_RS24250 are separated by 255 nucleotidesBPHYT_RS24250 and BPHYT_RS24255 are separated by 198 nucleotidesBPHYT_RS24255 and BPHYT_RS24260 are separated by 216 nucleotides BPHYT_RS24245: BPHYT_RS24245 - MerR family transcriptional regulator, at 1,005,743 to 1,006,609 _RS24245 BPHYT_RS24250: BPHYT_RS24250 - GCN5 family acetyltransferase, at 1,006,865 to 1,007,458 _RS24250 BPHYT_RS24255: BPHYT_RS24255 - hypothetical protein, at 1,007,657 to 1,007,935 _RS24255 BPHYT_RS24260: BPHYT_RS24260 - RpiR family transcriptional regulator, at 1,008,152 to 1,009,048 _RS24260 Position (kb) 1006 1007 1008Strain fitness (log2 ratio) -2 -1 0 1at 1006.301 kb on - strand, within BPHYT_RS24245at 1006.362 kb on + strand, within BPHYT_RS24245at 1006.419 kb on + strand, within BPHYT_RS24245at 1006.420 kb on - strand, within BPHYT_RS24245at 1006.531 kb on - strandat 1007.247 kb on + strand, within BPHYT_RS24250at 1007.247 kb on + strand, within BPHYT_RS24250at 1007.288 kb on + strand, within BPHYT_RS24250at 1007.353 kb on - strand, within BPHYT_RS24250at 1007.458 kb on + strandat 1007.459 kb on - strandat 1007.459 kb on - strandat 1007.499 kb on - strandat 1007.501 kb on + strandat 1007.502 kb on - strandat 1007.551 kb on + strandat 1007.599 kb on - strandat 1007.658 kb on + strandat 1007.659 kb on - strandat 1007.659 kb on - strandat 1007.663 kb on - strandat 1007.663 kb on - strandat 1007.671 kb on - strandat 1007.675 kb on - strandat 1007.713 kb on - strand, within BPHYT_RS24255at 1007.723 kb on - strand, within BPHYT_RS24255at 1007.880 kb on + strand, within BPHYT_RS24255at 1008.433 kb on - strand, within BPHYT_RS24260

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1
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1,006,301 - BPHYT_RS24245 0.64 -0.6
1,006,362 + BPHYT_RS24245 0.71 -0.2
1,006,419 + BPHYT_RS24245 0.78 -1.2
1,006,420 - BPHYT_RS24245 0.78 -0.3
1,006,531 - +0.3
1,007,247 + BPHYT_RS24250 0.64 -0.4
1,007,247 + BPHYT_RS24250 0.64 +0.3
1,007,288 + BPHYT_RS24250 0.71 +0.2
1,007,353 - BPHYT_RS24250 0.82 +0.3
1,007,458 + +0.4
1,007,459 - +0.4
1,007,459 - -1.0
1,007,499 - -1.1
1,007,501 + -0.6
1,007,502 - -0.6
1,007,551 + +0.8
1,007,599 - -1.6
1,007,658 + +0.2
1,007,659 - -0.1
1,007,659 - -0.3
1,007,663 - -1.2
1,007,663 - +0.5
1,007,671 - +0.8
1,007,675 - -2.2
1,007,713 - BPHYT_RS24255 0.20 -0.6
1,007,723 - BPHYT_RS24255 0.24 -0.1
1,007,880 + BPHYT_RS24255 0.80 -0.4
1,008,433 - BPHYT_RS24260 0.31 +0.6

Or see this region's nucleotide sequence