Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22390 and BPHYT_RS22395 are separated by 347 nucleotides BPHYT_RS22395 and BPHYT_RS22400 are separated by 181 nucleotides BPHYT_RS22400 and BPHYT_RS22405 are separated by 57 nucleotides
BPHYT_RS22390: BPHYT_RS22390 - glycine/betaine ABC transporter substrate-binding protein, at 579,184 to 580,149
_RS22390
BPHYT_RS22395: BPHYT_RS22395 - AraC family transcriptional regulator, at 580,497 to 581,450
_RS22395
BPHYT_RS22400: BPHYT_RS22400 - hypothetical protein, at 581,632 to 581,958
_RS22400
BPHYT_RS22405: BPHYT_RS22405 - HxlR family transcriptional regulator, at 582,016 to 582,417
_RS22405
Position (kb) 580
581
582 Strain fitness (log2 ratio) -2
-1
0
1 at 579.689 kb on + strand, within BPHYT_RS22390 at 579.762 kb on - strand, within BPHYT_RS22390 at 579.802 kb on - strand, within BPHYT_RS22390 at 580.282 kb on - strand at 580.391 kb on + strand at 580.391 kb on + strand at 580.508 kb on + strand at 580.508 kb on + strand at 580.743 kb on + strand, within BPHYT_RS22395 at 580.743 kb on + strand, within BPHYT_RS22395 at 580.958 kb on + strand, within BPHYT_RS22395 at 581.275 kb on - strand, within BPHYT_RS22395 at 581.547 kb on - strand at 581.548 kb on + strand at 581.552 kb on + strand at 581.553 kb on - strand at 581.553 kb on - strand at 581.562 kb on + strand at 581.794 kb on + strand, within BPHYT_RS22400 at 581.926 kb on + strand at 581.944 kb on - strand at 581.975 kb on + strand at 581.975 kb on + strand at 581.976 kb on - strand at 581.979 kb on - strand at 582.006 kb on - strand at 582.017 kb on + strand at 582.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 579,689 + BPHYT_RS22390 0.52 -1.2 579,762 - BPHYT_RS22390 0.60 -0.0 579,802 - BPHYT_RS22390 0.64 -0.5 580,282 - -0.7 580,391 + -0.6 580,391 + +0.4 580,508 + +0.4 580,508 + +0.1 580,743 + BPHYT_RS22395 0.26 -0.7 580,743 + BPHYT_RS22395 0.26 +0.5 580,958 + BPHYT_RS22395 0.48 -1.9 581,275 - BPHYT_RS22395 0.82 -0.2 581,547 - -1.0 581,548 + -1.4 581,552 + +0.2 581,553 - +0.0 581,553 - -0.3 581,562 + -0.8 581,794 + BPHYT_RS22400 0.50 +0.5 581,926 + -1.2 581,944 - +0.4 581,975 + -0.7 581,975 + -2.2 581,976 - -0.3 581,979 - +0.1 582,006 - -1.8 582,017 + +0.7 582,417 + -0.3
Or see this region's nucleotide sequence