Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14210

Experiment: 24hr PHYTO3 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14205 and BPHYT_RS14210 are separated by 156 nucleotidesBPHYT_RS14210 and BPHYT_RS14215 are separated by 19 nucleotidesBPHYT_RS14215 and BPHYT_RS14220 overlap by 4 nucleotides BPHYT_RS14205: BPHYT_RS14205 - lytic transglycosylase, at 3,246,270 to 3,247,964 _RS14205 BPHYT_RS14210: BPHYT_RS14210 - hydroxyacylglutathione hydrolase, at 3,248,121 to 3,248,912 _RS14210 BPHYT_RS14215: BPHYT_RS14215 - SAM-dependent methyltransferase, at 3,248,932 to 3,249,795 _RS14215 BPHYT_RS14220: BPHYT_RS14220 - ribonuclease H, at 3,249,792 to 3,250,238 _RS14220 Position (kb) 3248 3249Strain fitness (log2 ratio) -2 -1 0 1 2at 3247.121 kb on + strand, within BPHYT_RS14205at 3247.199 kb on - strand, within BPHYT_RS14205at 3247.249 kb on + strand, within BPHYT_RS14205at 3247.253 kb on + strand, within BPHYT_RS14205at 3247.352 kb on + strand, within BPHYT_RS14205at 3247.353 kb on - strand, within BPHYT_RS14205at 3247.354 kb on + strand, within BPHYT_RS14205at 3247.480 kb on + strand, within BPHYT_RS14205at 3247.480 kb on + strand, within BPHYT_RS14205at 3247.481 kb on - strand, within BPHYT_RS14205at 3247.627 kb on + strand, within BPHYT_RS14205at 3247.627 kb on + strand, within BPHYT_RS14205at 3247.634 kb on - strand, within BPHYT_RS14205at 3247.666 kb on + strand, within BPHYT_RS14205at 3247.684 kb on + strand, within BPHYT_RS14205at 3247.684 kb on + strand, within BPHYT_RS14205at 3247.852 kb on - strandat 3247.949 kb on + strandat 3247.949 kb on + strandat 3247.950 kb on - strandat 3247.950 kb on - strandat 3247.956 kb on - strandat 3247.956 kb on - strandat 3247.956 kb on - strandat 3248.008 kb on + strandat 3248.103 kb on - strandat 3248.103 kb on - strandat 3248.157 kb on - strandat 3248.157 kb on - strandat 3248.387 kb on - strand, within BPHYT_RS14210at 3248.387 kb on - strand, within BPHYT_RS14210at 3248.758 kb on - strand, within BPHYT_RS14210at 3248.794 kb on + strand, within BPHYT_RS14210at 3248.795 kb on - strand, within BPHYT_RS14210at 3248.795 kb on - strand, within BPHYT_RS14210at 3248.866 kb on + strandat 3248.867 kb on - strandat 3248.867 kb on - strandat 3248.867 kb on - strandat 3248.867 kb on - strandat 3248.892 kb on + strandat 3248.897 kb on - strandat 3248.932 kb on - strandat 3249.774 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1
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3,247,121 + BPHYT_RS14205 0.50 +2.2
3,247,199 - BPHYT_RS14205 0.55 -1.3
3,247,249 + BPHYT_RS14205 0.58 +1.4
3,247,253 + BPHYT_RS14205 0.58 -0.4
3,247,352 + BPHYT_RS14205 0.64 -0.7
3,247,353 - BPHYT_RS14205 0.64 -0.6
3,247,354 + BPHYT_RS14205 0.64 -1.9
3,247,480 + BPHYT_RS14205 0.71 -0.3
3,247,480 + BPHYT_RS14205 0.71 -0.5
3,247,481 - BPHYT_RS14205 0.71 -0.8
3,247,627 + BPHYT_RS14205 0.80 -2.2
3,247,627 + BPHYT_RS14205 0.80 -1.0
3,247,634 - BPHYT_RS14205 0.80 -0.1
3,247,666 + BPHYT_RS14205 0.82 -1.3
3,247,684 + BPHYT_RS14205 0.83 +0.5
3,247,684 + BPHYT_RS14205 0.83 -1.4
3,247,852 - -1.2
3,247,949 + +1.1
3,247,949 + +1.2
3,247,950 - +0.1
3,247,950 - -0.5
3,247,956 - +0.5
3,247,956 - -1.2
3,247,956 - -0.4
3,248,008 + -1.1
3,248,103 - +0.2
3,248,103 - +0.0
3,248,157 - +0.2
3,248,157 - +1.0
3,248,387 - BPHYT_RS14210 0.34 -0.5
3,248,387 - BPHYT_RS14210 0.34 -1.3
3,248,758 - BPHYT_RS14210 0.80 +0.1
3,248,794 + BPHYT_RS14210 0.85 -0.9
3,248,795 - BPHYT_RS14210 0.85 -0.5
3,248,795 - BPHYT_RS14210 0.85 -0.7
3,248,866 + -0.3
3,248,867 - -1.1
3,248,867 - +0.3
3,248,867 - +0.5
3,248,867 - -0.0
3,248,892 + +0.4
3,248,897 - -0.3
3,248,932 - +0.5
3,249,774 + +0.4

Or see this region's nucleotide sequence