Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14205 and BPHYT_RS14210 are separated by 156 nucleotides BPHYT_RS14210 and BPHYT_RS14215 are separated by 19 nucleotides BPHYT_RS14215 and BPHYT_RS14220 overlap by 4 nucleotides
BPHYT_RS14205: BPHYT_RS14205 - lytic transglycosylase, at 3,246,270 to 3,247,964
_RS14205
BPHYT_RS14210: BPHYT_RS14210 - hydroxyacylglutathione hydrolase, at 3,248,121 to 3,248,912
_RS14210
BPHYT_RS14215: BPHYT_RS14215 - SAM-dependent methyltransferase, at 3,248,932 to 3,249,795
_RS14215
BPHYT_RS14220: BPHYT_RS14220 - ribonuclease H, at 3,249,792 to 3,250,238
_RS14220
Position (kb)
3248
3249 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3247.121 kb on + strand, within BPHYT_RS14205 at 3247.199 kb on - strand, within BPHYT_RS14205 at 3247.249 kb on + strand, within BPHYT_RS14205 at 3247.253 kb on + strand, within BPHYT_RS14205 at 3247.352 kb on + strand, within BPHYT_RS14205 at 3247.353 kb on - strand, within BPHYT_RS14205 at 3247.354 kb on + strand, within BPHYT_RS14205 at 3247.480 kb on + strand, within BPHYT_RS14205 at 3247.480 kb on + strand, within BPHYT_RS14205 at 3247.481 kb on - strand, within BPHYT_RS14205 at 3247.627 kb on + strand, within BPHYT_RS14205 at 3247.627 kb on + strand, within BPHYT_RS14205 at 3247.634 kb on - strand, within BPHYT_RS14205 at 3247.666 kb on + strand, within BPHYT_RS14205 at 3247.684 kb on + strand, within BPHYT_RS14205 at 3247.684 kb on + strand, within BPHYT_RS14205 at 3247.852 kb on - strand at 3247.949 kb on + strand at 3247.949 kb on + strand at 3247.950 kb on - strand at 3247.950 kb on - strand at 3247.956 kb on - strand at 3247.956 kb on - strand at 3247.956 kb on - strand at 3248.008 kb on + strand at 3248.103 kb on - strand at 3248.103 kb on - strand at 3248.157 kb on - strand at 3248.157 kb on - strand at 3248.387 kb on - strand, within BPHYT_RS14210 at 3248.387 kb on - strand, within BPHYT_RS14210 at 3248.758 kb on - strand, within BPHYT_RS14210 at 3248.794 kb on + strand, within BPHYT_RS14210 at 3248.795 kb on - strand, within BPHYT_RS14210 at 3248.795 kb on - strand, within BPHYT_RS14210 at 3248.866 kb on + strand at 3248.867 kb on - strand at 3248.867 kb on - strand at 3248.867 kb on - strand at 3248.867 kb on - strand at 3248.892 kb on + strand at 3248.897 kb on - strand at 3248.932 kb on - strand at 3249.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 3,247,121 + BPHYT_RS14205 0.50 +2.2 3,247,199 - BPHYT_RS14205 0.55 -1.3 3,247,249 + BPHYT_RS14205 0.58 +1.4 3,247,253 + BPHYT_RS14205 0.58 -0.4 3,247,352 + BPHYT_RS14205 0.64 -0.7 3,247,353 - BPHYT_RS14205 0.64 -0.6 3,247,354 + BPHYT_RS14205 0.64 -1.9 3,247,480 + BPHYT_RS14205 0.71 -0.3 3,247,480 + BPHYT_RS14205 0.71 -0.5 3,247,481 - BPHYT_RS14205 0.71 -0.8 3,247,627 + BPHYT_RS14205 0.80 -2.2 3,247,627 + BPHYT_RS14205 0.80 -1.0 3,247,634 - BPHYT_RS14205 0.80 -0.1 3,247,666 + BPHYT_RS14205 0.82 -1.3 3,247,684 + BPHYT_RS14205 0.83 +0.5 3,247,684 + BPHYT_RS14205 0.83 -1.4 3,247,852 - -1.2 3,247,949 + +1.1 3,247,949 + +1.2 3,247,950 - +0.1 3,247,950 - -0.5 3,247,956 - +0.5 3,247,956 - -1.2 3,247,956 - -0.4 3,248,008 + -1.1 3,248,103 - +0.2 3,248,103 - +0.0 3,248,157 - +0.2 3,248,157 - +1.0 3,248,387 - BPHYT_RS14210 0.34 -0.5 3,248,387 - BPHYT_RS14210 0.34 -1.3 3,248,758 - BPHYT_RS14210 0.80 +0.1 3,248,794 + BPHYT_RS14210 0.85 -0.9 3,248,795 - BPHYT_RS14210 0.85 -0.5 3,248,795 - BPHYT_RS14210 0.85 -0.7 3,248,866 + -0.3 3,248,867 - -1.1 3,248,867 - +0.3 3,248,867 - +0.5 3,248,867 - -0.0 3,248,892 + +0.4 3,248,897 - -0.3 3,248,932 - +0.5 3,249,774 + +0.4
Or see this region's nucleotide sequence