Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10115 and BPHYT_RS10120 are separated by 275 nucleotides BPHYT_RS10120 and BPHYT_RS10125 are separated by 160 nucleotides BPHYT_RS10125 and BPHYT_RS10130 are separated by 711 nucleotides BPHYT_RS10130 and BPHYT_RS10135 are separated by 35 nucleotides
BPHYT_RS10115: BPHYT_RS10115 - hypothetical protein, at 2,304,178 to 2,304,921
_RS10115
BPHYT_RS10120: BPHYT_RS10120 - membrane protein, at 2,305,197 to 2,305,748
_RS10120
BPHYT_RS10125: BPHYT_RS10125 - hypothetical protein, at 2,305,909 to 2,306,361
_RS10125
BPHYT_RS10130: BPHYT_RS10130 - hypothetical protein, at 2,307,073 to 2,307,252
_RS10130
BPHYT_RS10135: BPHYT_RS10135 - hypothetical protein, at 2,307,288 to 2,307,713
_RS10135
Position (kb)
2305
2306
2307 Strain fitness (log2 ratio)
-1
0
1 at 2304.998 kb on + strand at 2304.998 kb on + strand at 2304.999 kb on - strand at 2305.102 kb on + strand at 2305.102 kb on + strand at 2305.196 kb on + strand at 2305.261 kb on + strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.806 kb on + strand at 2306.194 kb on + strand, within BPHYT_RS10125 at 2306.478 kb on - strand at 2306.478 kb on - strand at 2306.702 kb on - strand at 2306.846 kb on + strand at 2307.012 kb on + strand at 2307.031 kb on - strand at 2307.119 kb on + strand, within BPHYT_RS10130 at 2307.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 2,304,998 + -1.1 2,304,998 + -0.8 2,304,999 - -1.1 2,305,102 + -0.1 2,305,102 + +0.3 2,305,196 + +0.4 2,305,261 + BPHYT_RS10120 0.12 -0.1 2,305,262 - BPHYT_RS10120 0.12 -0.3 2,305,262 - BPHYT_RS10120 0.12 -0.5 2,305,262 - BPHYT_RS10120 0.12 +0.9 2,305,806 + -1.1 2,306,194 + BPHYT_RS10125 0.63 -1.0 2,306,478 - -0.1 2,306,478 - -0.3 2,306,702 - -0.0 2,306,846 + +0.5 2,307,012 + -0.6 2,307,031 - -0.5 2,307,119 + BPHYT_RS10130 0.26 +1.1 2,307,247 - +0.0
Or see this region's nucleotide sequence