Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09860 and BPHYT_RS09865 are separated by 60 nucleotides BPHYT_RS09865 and BPHYT_RS09870 are separated by 143 nucleotides BPHYT_RS09870 and BPHYT_RS09875 are separated by 285 nucleotides
BPHYT_RS09860: BPHYT_RS09860 - pilus assembly protein PilP, at 2,243,674 to 2,244,348
_RS09860
BPHYT_RS09865: BPHYT_RS09865 - methyltransferase, at 2,244,409 to 2,244,897
_RS09865
BPHYT_RS09870: BPHYT_RS09870 - membrane protein, at 2,245,041 to 2,246,198
_RS09870
BPHYT_RS09875: BPHYT_RS09875 - aldehyde dehydrogenase, at 2,246,484 to 2,247,929
_RS09875
Position (kb)
2245
2246
2247 Strain fitness (log2 ratio)
-1
0
1 at 2244.110 kb on - strand, within BPHYT_RS09860 at 2245.115 kb on + strand at 2245.115 kb on + strand at 2245.166 kb on - strand, within BPHYT_RS09870 at 2245.283 kb on - strand, within BPHYT_RS09870 at 2245.503 kb on - strand, within BPHYT_RS09870 at 2245.577 kb on + strand, within BPHYT_RS09870 at 2245.579 kb on + strand, within BPHYT_RS09870 at 2245.823 kb on - strand, within BPHYT_RS09870 at 2245.841 kb on - strand, within BPHYT_RS09870 at 2246.219 kb on - strand at 2246.947 kb on - strand, within BPHYT_RS09875 at 2247.128 kb on - strand, within BPHYT_RS09875
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 2,244,110 - BPHYT_RS09860 0.65 +0.6 2,245,115 + +0.5 2,245,115 + -0.1 2,245,166 - BPHYT_RS09870 0.11 +0.5 2,245,283 - BPHYT_RS09870 0.21 -1.3 2,245,503 - BPHYT_RS09870 0.40 +1.3 2,245,577 + BPHYT_RS09870 0.46 -1.0 2,245,579 + BPHYT_RS09870 0.46 -0.6 2,245,823 - BPHYT_RS09870 0.68 -0.8 2,245,841 - BPHYT_RS09870 0.69 -0.7 2,246,219 - +0.4 2,246,947 - BPHYT_RS09875 0.32 -1.3 2,247,128 - BPHYT_RS09875 0.45 +0.5
Or see this region's nucleotide sequence