Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04930 and BPHYT_RS04935 are separated by 4 nucleotides BPHYT_RS04935 and BPHYT_RS04940 are separated by 72 nucleotides BPHYT_RS04940 and BPHYT_RS04945 are separated by 22 nucleotides
BPHYT_RS04930: BPHYT_RS04930 - segregation and condensation protein A, at 1,131,909 to 1,132,784
_RS04930
BPHYT_RS04935: BPHYT_RS04935 - hypothetical protein, at 1,132,789 to 1,132,974
_RS04935
BPHYT_RS04940: BPHYT_RS04940 - hypothetical protein, at 1,133,047 to 1,133,277
_RS04940
BPHYT_RS04945: BPHYT_RS04945 - membrane protein, at 1,133,300 to 1,135,135
_RS04945
Position (kb)
1133
1134 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1133.049 kb on - strand at 1133.124 kb on + strand, within BPHYT_RS04940 at 1133.234 kb on - strand, within BPHYT_RS04940 at 1133.234 kb on - strand, within BPHYT_RS04940 at 1133.234 kb on - strand, within BPHYT_RS04940 at 1133.376 kb on + strand at 1133.469 kb on - strand at 1133.633 kb on + strand, within BPHYT_RS04945 at 1133.634 kb on - strand, within BPHYT_RS04945 at 1133.634 kb on - strand, within BPHYT_RS04945 at 1133.665 kb on + strand, within BPHYT_RS04945 at 1133.888 kb on + strand, within BPHYT_RS04945 at 1133.889 kb on - strand, within BPHYT_RS04945 at 1133.906 kb on + strand, within BPHYT_RS04945 at 1133.906 kb on + strand, within BPHYT_RS04945 at 1133.906 kb on + strand, within BPHYT_RS04945 at 1133.907 kb on - strand, within BPHYT_RS04945 at 1133.907 kb on - strand, within BPHYT_RS04945 at 1133.963 kb on + strand, within BPHYT_RS04945 at 1133.963 kb on + strand, within BPHYT_RS04945 at 1133.963 kb on + strand, within BPHYT_RS04945 at 1133.964 kb on - strand, within BPHYT_RS04945 at 1133.964 kb on - strand, within BPHYT_RS04945 at 1134.003 kb on + strand, within BPHYT_RS04945 at 1134.004 kb on - strand, within BPHYT_RS04945 at 1134.071 kb on + strand, within BPHYT_RS04945 at 1134.125 kb on + strand, within BPHYT_RS04945 at 1134.125 kb on + strand, within BPHYT_RS04945 at 1134.203 kb on + strand, within BPHYT_RS04945 at 1134.204 kb on - strand, within BPHYT_RS04945 at 1134.204 kb on - strand, within BPHYT_RS04945 at 1134.204 kb on - strand, within BPHYT_RS04945 at 1134.210 kb on - strand, within BPHYT_RS04945 at 1134.210 kb on - strand at 1134.210 kb on - strand, within BPHYT_RS04945 at 1134.210 kb on - strand, within BPHYT_RS04945 at 1134.236 kb on + strand at 1134.272 kb on + strand, within BPHYT_RS04945 at 1134.272 kb on + strand, within BPHYT_RS04945 at 1134.272 kb on + strand, within BPHYT_RS04945 at 1134.272 kb on + strand, within BPHYT_RS04945 at 1134.272 kb on + strand, within BPHYT_RS04945 at 1134.272 kb on + strand, within BPHYT_RS04945
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 1,133,049 - +0.7 1,133,124 + BPHYT_RS04940 0.33 -0.3 1,133,234 - BPHYT_RS04940 0.81 +2.5 1,133,234 - BPHYT_RS04940 0.81 +2.2 1,133,234 - BPHYT_RS04940 0.81 +2.0 1,133,376 + +0.5 1,133,469 - +2.2 1,133,633 + BPHYT_RS04945 0.18 +1.8 1,133,634 - BPHYT_RS04945 0.18 +2.5 1,133,634 - BPHYT_RS04945 0.18 +2.2 1,133,665 + BPHYT_RS04945 0.20 +1.4 1,133,888 + BPHYT_RS04945 0.32 +2.4 1,133,889 - BPHYT_RS04945 0.32 +1.8 1,133,906 + BPHYT_RS04945 0.33 +2.4 1,133,906 + BPHYT_RS04945 0.33 +1.7 1,133,906 + BPHYT_RS04945 0.33 +2.3 1,133,907 - BPHYT_RS04945 0.33 +2.6 1,133,907 - BPHYT_RS04945 0.33 +3.5 1,133,963 + BPHYT_RS04945 0.36 +2.3 1,133,963 + BPHYT_RS04945 0.36 +1.7 1,133,963 + BPHYT_RS04945 0.36 +0.5 1,133,964 - BPHYT_RS04945 0.36 +1.8 1,133,964 - BPHYT_RS04945 0.36 +1.7 1,134,003 + BPHYT_RS04945 0.38 +1.6 1,134,004 - BPHYT_RS04945 0.38 +2.2 1,134,071 + BPHYT_RS04945 0.42 +2.3 1,134,125 + BPHYT_RS04945 0.45 +2.2 1,134,125 + BPHYT_RS04945 0.45 +1.8 1,134,203 + BPHYT_RS04945 0.49 +2.6 1,134,204 - BPHYT_RS04945 0.49 +2.1 1,134,204 - BPHYT_RS04945 0.49 +1.3 1,134,204 - BPHYT_RS04945 0.49 +2.4 1,134,210 - BPHYT_RS04945 0.50 +1.9 1,134,210 - +3.9 1,134,210 - BPHYT_RS04945 0.50 +2.1 1,134,210 - BPHYT_RS04945 0.50 +1.7 1,134,236 + +3.2 1,134,272 + BPHYT_RS04945 0.53 +2.2 1,134,272 + BPHYT_RS04945 0.53 +1.5 1,134,272 + BPHYT_RS04945 0.53 +1.8 1,134,272 + BPHYT_RS04945 0.53 +1.5 1,134,272 + BPHYT_RS04945 0.53 +2.0 1,134,272 + BPHYT_RS04945 0.53 +2.4
Or see this region's nucleotide sequence