Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01685 and BPHYT_RS01690 are separated by 89 nucleotides BPHYT_RS01690 and BPHYT_RS01695 are separated by 215 nucleotides BPHYT_RS01695 and BPHYT_RS01700 are separated by 65 nucleotides BPHYT_RS01700 and BPHYT_RS01705 are separated by 272 nucleotides
BPHYT_RS01685: BPHYT_RS01685 - phytanoyl-CoA dioxygenase, at 382,269 to 383,030
_RS01685
BPHYT_RS01690: BPHYT_RS01690 - membrane protein, at 383,120 to 383,326
_RS01690
BPHYT_RS01695: BPHYT_RS01695 - L-glyceraldehyde 3-phosphate reductase, at 383,542 to 384,585
_RS01695
BPHYT_RS01700: BPHYT_RS01700 - membrane protein, at 384,651 to 384,836
_RS01700
BPHYT_RS01705: BPHYT_RS01705 - MFS transporter, at 385,109 to 386,311
_RS01705
Position (kb) 383
384
385 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 382.566 kb on + strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.567 kb on - strand, within BPHYT_RS01685 at 382.605 kb on - strand, within BPHYT_RS01685 at 382.633 kb on - strand, within BPHYT_RS01685 at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.957 kb on - strand at 382.963 kb on - strand at 382.963 kb on - strand at 383.222 kb on - strand, within BPHYT_RS01690 at 383.274 kb on + strand, within BPHYT_RS01690 at 383.328 kb on + strand at 383.328 kb on + strand at 383.329 kb on - strand at 383.329 kb on - strand at 383.329 kb on - strand at 383.431 kb on + strand at 383.432 kb on - strand at 383.432 kb on - strand at 383.537 kb on + strand at 383.537 kb on + strand at 383.538 kb on - strand at 383.569 kb on + strand at 383.585 kb on - strand at 383.709 kb on + strand, within BPHYT_RS01695 at 383.737 kb on + strand, within BPHYT_RS01695 at 383.737 kb on + strand, within BPHYT_RS01695 at 383.935 kb on + strand, within BPHYT_RS01695 at 383.950 kb on + strand, within BPHYT_RS01695 at 383.951 kb on - strand, within BPHYT_RS01695 at 384.034 kb on + strand, within BPHYT_RS01695 at 384.097 kb on + strand, within BPHYT_RS01695 at 384.130 kb on + strand, within BPHYT_RS01695 at 384.241 kb on + strand, within BPHYT_RS01695 at 384.242 kb on - strand, within BPHYT_RS01695 at 384.826 kb on + strand at 384.826 kb on + strand at 384.848 kb on + strand at 384.848 kb on + strand at 384.849 kb on - strand at 384.849 kb on - strand at 384.879 kb on + strand at 384.880 kb on - strand at 384.914 kb on - strand at 384.915 kb on + strand at 384.916 kb on - strand at 384.916 kb on - strand at 384.936 kb on - strand at 384.936 kb on - strand at 384.937 kb on + strand at 385.053 kb on + strand at 385.054 kb on - strand at 385.231 kb on - strand, within BPHYT_RS01705 at 385.257 kb on + strand, within BPHYT_RS01705 at 385.258 kb on - strand, within BPHYT_RS01705 at 385.260 kb on + strand, within BPHYT_RS01705 at 385.272 kb on + strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 382,566 + BPHYT_RS01685 0.39 -1.4 382,567 - BPHYT_RS01685 0.39 +0.0 382,567 - BPHYT_RS01685 0.39 -0.1 382,567 - BPHYT_RS01685 0.39 +1.0 382,567 - BPHYT_RS01685 0.39 -0.9 382,605 - BPHYT_RS01685 0.44 -1.1 382,633 - BPHYT_RS01685 0.48 +1.3 382,957 - +3.4 382,957 - -1.0 382,957 - +0.4 382,957 - +1.8 382,957 - +0.0 382,963 - +0.5 382,963 - +0.6 383,222 - BPHYT_RS01690 0.49 +1.1 383,274 + BPHYT_RS01690 0.74 -0.7 383,328 + +0.5 383,328 + +1.7 383,329 - +0.4 383,329 - -0.4 383,329 - +1.0 383,431 + -1.3 383,432 - -0.6 383,432 - -1.0 383,537 + +0.0 383,537 + -0.1 383,538 - +1.2 383,569 + +1.2 383,585 - +0.8 383,709 + BPHYT_RS01695 0.16 -0.3 383,737 + BPHYT_RS01695 0.19 +1.2 383,737 + BPHYT_RS01695 0.19 -0.8 383,935 + BPHYT_RS01695 0.38 -1.4 383,950 + BPHYT_RS01695 0.39 +2.5 383,951 - BPHYT_RS01695 0.39 -1.4 384,034 + BPHYT_RS01695 0.47 -1.6 384,097 + BPHYT_RS01695 0.53 +0.9 384,130 + BPHYT_RS01695 0.56 -2.3 384,241 + BPHYT_RS01695 0.67 -0.9 384,242 - BPHYT_RS01695 0.67 -1.8 384,826 + +0.3 384,826 + -0.0 384,848 + +1.1 384,848 + -0.9 384,849 - +0.5 384,849 - -0.3 384,879 + -0.1 384,880 - -0.0 384,914 - +0.0 384,915 + -1.1 384,916 - +0.0 384,916 - -0.4 384,936 - +0.5 384,936 - -0.2 384,937 + -1.8 385,053 + +0.5 385,054 - -0.2 385,231 - BPHYT_RS01705 0.10 +0.6 385,257 + BPHYT_RS01705 0.12 +1.0 385,258 - BPHYT_RS01705 0.12 -0.7 385,260 + BPHYT_RS01705 0.13 -1.6 385,272 + BPHYT_RS01705 0.14 -0.5 385,519 - BPHYT_RS01705 0.34 +2.0 385,519 - BPHYT_RS01705 0.34 -0.9 385,519 - BPHYT_RS01705 0.34 +0.6 385,519 - BPHYT_RS01705 0.34 +0.3 385,560 + BPHYT_RS01705 0.37 -0.3 385,560 + BPHYT_RS01705 0.37 -1.1 385,561 - BPHYT_RS01705 0.38 +0.0 385,561 - BPHYT_RS01705 0.38 -1.1
Or see this region's nucleotide sequence