Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01055 and BPHYT_RS01060 are separated by 79 nucleotides BPHYT_RS01060 and BPHYT_RS01065 are separated by 161 nucleotides
BPHYT_RS01055: BPHYT_RS01055 - LysR family transcriptional regulator, at 240,736 to 241,665
_RS01055
BPHYT_RS01060: BPHYT_RS01060 - alcohol dehydrogenase, at 241,745 to 242,998
_RS01060
BPHYT_RS01065: BPHYT_RS01065 - GMC family oxidoreductase, at 243,160 to 244,938
_RS01065
Position (kb) 241
242
243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 240.750 kb on - strand at 240.768 kb on + strand at 241.096 kb on + strand, within BPHYT_RS01055 at 241.182 kb on + strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.244 kb on - strand, within BPHYT_RS01055 at 241.557 kb on + strand, within BPHYT_RS01055 at 241.687 kb on + strand at 241.687 kb on + strand at 241.688 kb on - strand at 242.077 kb on - strand, within BPHYT_RS01060 at 242.077 kb on - strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.557 kb on + strand, within BPHYT_RS01060 at 242.602 kb on - strand, within BPHYT_RS01060 at 242.605 kb on + strand, within BPHYT_RS01060 at 242.862 kb on + strand, within BPHYT_RS01060 at 242.863 kb on - strand, within BPHYT_RS01060 at 242.885 kb on - strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.140 kb on - strand at 243.140 kb on - strand at 243.195 kb on - strand at 243.224 kb on + strand at 243.257 kb on + strand at 243.510 kb on - strand, within BPHYT_RS01065 at 243.602 kb on + strand, within BPHYT_RS01065 at 243.647 kb on + strand, within BPHYT_RS01065 at 243.648 kb on - strand, within BPHYT_RS01065 at 243.753 kb on - strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.873 kb on - strand, within BPHYT_RS01065 at 243.947 kb on + strand, within BPHYT_RS01065 at 243.948 kb on - strand, within BPHYT_RS01065
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 240,750 - -0.3 240,768 + -0.6 241,096 + BPHYT_RS01055 0.39 -0.0 241,182 + BPHYT_RS01055 0.48 +0.1 241,183 - BPHYT_RS01055 0.48 -0.3 241,183 - BPHYT_RS01055 0.48 -0.1 241,183 - BPHYT_RS01055 0.48 -0.1 241,244 - BPHYT_RS01055 0.55 -1.0 241,557 + BPHYT_RS01055 0.88 -0.2 241,687 + -0.6 241,687 + +0.1 241,688 - +0.1 242,077 - BPHYT_RS01060 0.26 -1.0 242,077 - BPHYT_RS01060 0.26 -0.6 242,367 + BPHYT_RS01060 0.50 -0.1 242,367 + BPHYT_RS01060 0.50 +0.2 242,367 + BPHYT_RS01060 0.50 +1.0 242,557 + BPHYT_RS01060 0.65 +1.0 242,602 - BPHYT_RS01060 0.68 +0.2 242,605 + BPHYT_RS01060 0.69 -0.1 242,862 + BPHYT_RS01060 0.89 -1.0 242,863 - BPHYT_RS01060 0.89 +0.7 242,885 - -0.4 243,139 + +0.5 243,139 + -0.1 243,139 + -0.6 243,140 - -0.5 243,140 - -2.8 243,195 - -0.4 243,224 + +0.6 243,257 + +0.2 243,510 - BPHYT_RS01065 0.20 -0.5 243,602 + BPHYT_RS01065 0.25 -1.1 243,647 + BPHYT_RS01065 0.27 +0.3 243,648 - BPHYT_RS01065 0.27 -2.2 243,753 - BPHYT_RS01065 0.33 -1.0 243,872 + BPHYT_RS01065 0.40 +2.5 243,872 + BPHYT_RS01065 0.40 +0.6 243,872 + BPHYT_RS01065 0.40 +2.2 243,873 - BPHYT_RS01065 0.40 -0.5 243,947 + BPHYT_RS01065 0.44 -2.0 243,948 - BPHYT_RS01065 0.44 +1.5
Or see this region's nucleotide sequence