Experiment: 24hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00715 and BPHYT_RS00720 are separated by 54 nucleotides BPHYT_RS00720 and BPHYT_RS00725 overlap by 4 nucleotides BPHYT_RS00725 and BPHYT_RS00730 are separated by 81 nucleotides
BPHYT_RS00715: BPHYT_RS00715 - branched-chain amino acid ABC transporter substrate-binding protein, at 166,337 to 167,515
_RS00715
BPHYT_RS00720: BPHYT_RS00720 - amidase, at 167,570 to 168,997
_RS00720
BPHYT_RS00725: BPHYT_RS00725 - hypothetical protein, at 168,994 to 169,539
_RS00725
BPHYT_RS00730: BPHYT_RS00730 - porin, at 169,621 to 170,655
_RS00730
Position (kb) 167
168
169 Strain fitness (log2 ratio) -1
0
1 at 166.648 kb on + strand, within BPHYT_RS00715 at 167.157 kb on - strand, within BPHYT_RS00715 at 167.205 kb on - strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.216 kb on + strand, within BPHYT_RS00715 at 167.283 kb on - strand, within BPHYT_RS00715 at 167.517 kb on + strand at 167.517 kb on + strand at 167.517 kb on + strand at 167.659 kb on - strand at 168.492 kb on - strand, within BPHYT_RS00720 at 168.495 kb on - strand, within BPHYT_RS00720 at 168.695 kb on - strand, within BPHYT_RS00720 at 168.851 kb on - strand, within BPHYT_RS00720 at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 168.921 kb on - strand at 169.287 kb on - strand, within BPHYT_RS00725 at 169.482 kb on - strand, within BPHYT_RS00725 at 169.616 kb on + strand at 169.617 kb on - strand at 169.624 kb on + strand at 169.624 kb on + strand at 169.718 kb on + strand at 169.912 kb on + strand, within BPHYT_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 0.1 remove 166,648 + BPHYT_RS00715 0.26 -0.4 167,157 - BPHYT_RS00715 0.70 -0.2 167,205 - BPHYT_RS00715 0.74 -0.7 167,206 + BPHYT_RS00715 0.74 -0.4 167,206 + BPHYT_RS00715 0.74 -0.1 167,216 + BPHYT_RS00715 0.75 -0.4 167,283 - BPHYT_RS00715 0.80 -1.7 167,517 + +1.3 167,517 + +0.6 167,517 + +0.1 167,659 - +0.2 168,492 - BPHYT_RS00720 0.65 +0.1 168,495 - BPHYT_RS00720 0.65 -0.9 168,695 - BPHYT_RS00720 0.79 -1.7 168,851 - BPHYT_RS00720 0.90 -0.2 168,921 - +0.0 168,921 - -0.9 168,921 - +1.5 168,921 - +0.1 168,921 - -1.4 168,921 - -0.5 169,287 - BPHYT_RS00725 0.54 -0.4 169,482 - BPHYT_RS00725 0.89 +0.3 169,616 + -0.1 169,617 - -0.2 169,624 + -0.5 169,624 + -0.3 169,718 + -0.4 169,912 + BPHYT_RS00730 0.28 -1.5
Or see this region's nucleotide sequence