Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35215 and BPHYT_RS35220 are separated by 186 nucleotides BPHYT_RS35220 and BPHYT_RS35225 are separated by 4 nucleotides BPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotides BPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotides
BPHYT_RS35215: BPHYT_RS35215 - dehydrogenase, at 3,505,507 to 3,505,920
_RS35215
BPHYT_RS35220: BPHYT_RS35220 - extradiol dioxygenase, at 3,506,107 to 3,506,514
_RS35220
BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793
_RS35225
BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519
_RS35230
BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320
_RS35235
Position (kb)
3506
3507
3508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3505.743 kb on + strand, within BPHYT_RS35215 at 3506.113 kb on - strand at 3506.123 kb on - strand at 3506.246 kb on - strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.435 kb on - strand, within BPHYT_RS35220 at 3506.435 kb on - strand, within BPHYT_RS35220 at 3506.482 kb on + strand at 3506.483 kb on - strand at 3506.861 kb on - strand, within BPHYT_RS35225 at 3507.119 kb on + strand, within BPHYT_RS35225 at 3507.141 kb on - strand, within BPHYT_RS35225 at 3507.641 kb on + strand, within BPHYT_RS35225 at 3507.641 kb on + strand, within BPHYT_RS35225 at 3507.661 kb on - strand, within BPHYT_RS35225 at 3507.987 kb on + strand, within BPHYT_RS35230 at 3508.253 kb on - strand, within BPHYT_RS35230 at 3508.253 kb on - strand at 3508.579 kb on + strand at 3508.628 kb on - strand at 3508.698 kb on - strand at 3508.783 kb on - strand, within BPHYT_RS35235
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 3,505,743 + BPHYT_RS35215 0.57 +1.0 3,506,113 - -0.6 3,506,123 - -1.2 3,506,246 - BPHYT_RS35220 0.34 +0.3 3,506,434 + BPHYT_RS35220 0.80 -1.0 3,506,434 + BPHYT_RS35220 0.80 -0.1 3,506,434 + BPHYT_RS35220 0.80 +2.3 3,506,435 - BPHYT_RS35220 0.80 +0.3 3,506,435 - BPHYT_RS35220 0.80 +0.6 3,506,482 + -0.8 3,506,483 - -1.1 3,506,861 - BPHYT_RS35225 0.27 +0.1 3,507,119 + BPHYT_RS35225 0.47 -0.3 3,507,141 - BPHYT_RS35225 0.49 -0.7 3,507,641 + BPHYT_RS35225 0.88 -0.4 3,507,641 + BPHYT_RS35225 0.88 +0.3 3,507,661 - BPHYT_RS35225 0.90 -0.7 3,507,987 + BPHYT_RS35230 0.23 -1.4 3,508,253 - BPHYT_RS35230 0.61 -0.7 3,508,253 - +1.9 3,508,579 + +0.7 3,508,628 - -1.2 3,508,698 - -2.2 3,508,783 - BPHYT_RS35235 0.13 -0.1
Or see this region's nucleotide sequence