Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35225

Experiment: 24hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35215 and BPHYT_RS35220 are separated by 186 nucleotidesBPHYT_RS35220 and BPHYT_RS35225 are separated by 4 nucleotidesBPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotidesBPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotides BPHYT_RS35215: BPHYT_RS35215 - dehydrogenase, at 3,505,507 to 3,505,920 _RS35215 BPHYT_RS35220: BPHYT_RS35220 - extradiol dioxygenase, at 3,506,107 to 3,506,514 _RS35220 BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793 _RS35225 BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519 _RS35230 BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320 _RS35235 Position (kb) 3506 3507 3508Strain fitness (log2 ratio) -2 -1 0 1 2at 3505.743 kb on + strand, within BPHYT_RS35215at 3506.113 kb on - strandat 3506.123 kb on - strandat 3506.246 kb on - strand, within BPHYT_RS35220at 3506.434 kb on + strand, within BPHYT_RS35220at 3506.434 kb on + strand, within BPHYT_RS35220at 3506.434 kb on + strand, within BPHYT_RS35220at 3506.435 kb on - strand, within BPHYT_RS35220at 3506.435 kb on - strand, within BPHYT_RS35220at 3506.482 kb on + strandat 3506.483 kb on - strandat 3506.861 kb on - strand, within BPHYT_RS35225at 3507.119 kb on + strand, within BPHYT_RS35225at 3507.141 kb on - strand, within BPHYT_RS35225at 3507.641 kb on + strand, within BPHYT_RS35225at 3507.641 kb on + strand, within BPHYT_RS35225at 3507.661 kb on - strand, within BPHYT_RS35225at 3507.987 kb on + strand, within BPHYT_RS35230at 3508.253 kb on - strand, within BPHYT_RS35230at 3508.253 kb on - strandat 3508.579 kb on + strandat 3508.628 kb on - strandat 3508.698 kb on - strandat 3508.783 kb on - strand, within BPHYT_RS35235

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1
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3,505,743 + BPHYT_RS35215 0.57 +1.0
3,506,113 - -0.6
3,506,123 - -1.2
3,506,246 - BPHYT_RS35220 0.34 +0.3
3,506,434 + BPHYT_RS35220 0.80 -1.0
3,506,434 + BPHYT_RS35220 0.80 -0.1
3,506,434 + BPHYT_RS35220 0.80 +2.3
3,506,435 - BPHYT_RS35220 0.80 +0.3
3,506,435 - BPHYT_RS35220 0.80 +0.6
3,506,482 + -0.8
3,506,483 - -1.1
3,506,861 - BPHYT_RS35225 0.27 +0.1
3,507,119 + BPHYT_RS35225 0.47 -0.3
3,507,141 - BPHYT_RS35225 0.49 -0.7
3,507,641 + BPHYT_RS35225 0.88 -0.4
3,507,641 + BPHYT_RS35225 0.88 +0.3
3,507,661 - BPHYT_RS35225 0.90 -0.7
3,507,987 + BPHYT_RS35230 0.23 -1.4
3,508,253 - BPHYT_RS35230 0.61 -0.7
3,508,253 - +1.9
3,508,579 + +0.7
3,508,628 - -1.2
3,508,698 - -2.2
3,508,783 - BPHYT_RS35235 0.13 -0.1

Or see this region's nucleotide sequence