Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31015

Experiment: 24hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31010 and BPHYT_RS31015 overlap by 4 nucleotidesBPHYT_RS31015 and BPHYT_RS31020 overlap by 17 nucleotidesBPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotides BPHYT_RS31010: BPHYT_RS31010 - arabinose 5-phosphate isomerase, at 2,512,294 to 2,513,229 _RS31010 BPHYT_RS31015: BPHYT_RS31015 - 3-deoxy-manno-octulosonate cytidylyltransferase 2, at 2,513,226 to 2,514,017 _RS31015 BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555 _RS31020 BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412 _RS31025 Position (kb) 2513 2514 2515Strain fitness (log2 ratio) -1 0 1 2at 2513.214 kb on - strandat 2513.440 kb on + strand, within BPHYT_RS31015at 2513.441 kb on - strand, within BPHYT_RS31015at 2514.048 kb on - strandat 2514.237 kb on - strand, within BPHYT_RS31020

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1
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2,513,214 - +2.2
2,513,440 + BPHYT_RS31015 0.27 -0.5
2,513,441 - BPHYT_RS31015 0.27 -0.3
2,514,048 - -0.5
2,514,237 - BPHYT_RS31020 0.43 -1.7

Or see this region's nucleotide sequence