Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27650

Experiment: 24hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27640 and BPHYT_RS27645 are separated by 467 nucleotidesBPHYT_RS27645 and BPHYT_RS27650 are separated by 58 nucleotidesBPHYT_RS27650 and BPHYT_RS27655 are separated by 436 nucleotides BPHYT_RS27640: BPHYT_RS27640 - acetoacetyl-CoA reductase, at 1,769,304 to 1,770,050 _RS27640 BPHYT_RS27645: BPHYT_RS27645 - universal stress protein UspA, at 1,770,518 to 1,770,976 _RS27645 BPHYT_RS27650: BPHYT_RS27650 - hypothetical protein, at 1,771,035 to 1,771,382 _RS27650 BPHYT_RS27655: BPHYT_RS27655 - potassium transporter, at 1,771,819 to 1,772,652 _RS27655 Position (kb) 1771 1772Strain fitness (log2 ratio) -1 0 1at 1770.050 kb on + strandat 1770.051 kb on - strandat 1770.451 kb on - strandat 1770.505 kb on - strandat 1770.981 kb on - strandat 1771.459 kb on + strandat 1772.330 kb on - strand, within BPHYT_RS27655

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1
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1,770,050 + +0.1
1,770,051 - -0.8
1,770,451 - +0.2
1,770,505 - -0.1
1,770,981 - +0.8
1,771,459 + +0.1
1,772,330 - BPHYT_RS27655 0.61 -0.5

Or see this region's nucleotide sequence