Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27335 and BPHYT_RS27340 overlap by 4 nucleotides BPHYT_RS27340 and BPHYT_RS27345 overlap by 4 nucleotides BPHYT_RS27345 and BPHYT_RS27350 are separated by 184 nucleotides
BPHYT_RS27335: BPHYT_RS27335 - cytochrome C, at 1,707,461 to 1,708,738
_RS27335
BPHYT_RS27340: BPHYT_RS27340 - (2Fe-2S)-binding protein, at 1,708,735 to 1,709,247
_RS27340
BPHYT_RS27345: BPHYT_RS27345 - aldehyde dehydrogenase, at 1,709,244 to 1,711,508
_RS27345
BPHYT_RS27350: BPHYT_RS27350 - LysR family transcriptional regulator, at 1,711,693 to 1,712,598
_RS27350
Position (kb)
1709
1710
1711
1712 Strain fitness (log2 ratio)
-1
0
1
2 at 1708.593 kb on - strand, within BPHYT_RS27335 at 1708.616 kb on + strand at 1708.802 kb on - strand, within BPHYT_RS27340 at 1710.675 kb on - strand, within BPHYT_RS27345 at 1711.072 kb on - strand, within BPHYT_RS27345 at 1711.268 kb on + strand, within BPHYT_RS27345 at 1711.541 kb on + strand at 1711.600 kb on + strand at 1711.601 kb on - strand at 1711.761 kb on - strand at 1712.028 kb on - strand, within BPHYT_RS27350 at 1712.101 kb on + strand, within BPHYT_RS27350 at 1712.101 kb on + strand, within BPHYT_RS27350 at 1712.295 kb on + strand, within BPHYT_RS27350
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,708,593 - BPHYT_RS27335 0.89 -0.3 1,708,616 + -1.1 1,708,802 - BPHYT_RS27340 0.13 +2.6 1,710,675 - BPHYT_RS27345 0.63 -1.5 1,711,072 - BPHYT_RS27345 0.81 -1.0 1,711,268 + BPHYT_RS27345 0.89 +1.8 1,711,541 + +0.9 1,711,600 + +1.8 1,711,601 - -1.6 1,711,761 - +0.3 1,712,028 - BPHYT_RS27350 0.37 +0.3 1,712,101 + BPHYT_RS27350 0.45 +0.1 1,712,101 + BPHYT_RS27350 0.45 +0.3 1,712,295 + BPHYT_RS27350 0.66 -0.3
Or see this region's nucleotide sequence