Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27040 and BPHYT_RS27045 are separated by 132 nucleotides BPHYT_RS27045 and BPHYT_RS27050 are separated by 209 nucleotides BPHYT_RS27050 and BPHYT_RS27055 are separated by 90 nucleotides BPHYT_RS27055 and BPHYT_RS27060 are separated by 347 nucleotides
BPHYT_RS27040: BPHYT_RS27040 - permease, at 1,642,128 to 1,642,679
_RS27040
BPHYT_RS27045: BPHYT_RS27045 - hypothetical protein, at 1,642,812 to 1,643,153
_RS27045
BPHYT_RS27050: BPHYT_RS27050 - DNA topoisomerase, at 1,643,363 to 1,644,682
_RS27050
BPHYT_RS27055: BPHYT_RS27055 - hypothetical protein, at 1,644,773 to 1,645,066
_RS27055
BPHYT_RS27060: BPHYT_RS27060 - photosystem reaction center subunit H, at 1,645,414 to 1,645,911
_RS27060
Position (kb)
1643
1644
1645 Strain fitness (log2 ratio)
-2
-1
0
1 at 1642.462 kb on + strand, within BPHYT_RS27040 at 1642.644 kb on + strand at 1642.683 kb on + strand at 1643.675 kb on + strand, within BPHYT_RS27050 at 1644.080 kb on - strand, within BPHYT_RS27050 at 1644.116 kb on + strand, within BPHYT_RS27050 at 1644.308 kb on + strand, within BPHYT_RS27050 at 1644.787 kb on + strand at 1645.082 kb on + strand at 1645.142 kb on + strand at 1645.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,642,462 + BPHYT_RS27040 0.61 -0.2 1,642,644 + -1.2 1,642,683 + -1.4 1,643,675 + BPHYT_RS27050 0.24 +0.0 1,644,080 - BPHYT_RS27050 0.54 +1.6 1,644,116 + BPHYT_RS27050 0.57 -1.5 1,644,308 + BPHYT_RS27050 0.72 -0.8 1,644,787 + -1.0 1,645,082 + -0.2 1,645,142 + +0.4 1,645,142 + -1.9
Or see this region's nucleotide sequence