Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27050

Experiment: 24hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27040 and BPHYT_RS27045 are separated by 132 nucleotidesBPHYT_RS27045 and BPHYT_RS27050 are separated by 209 nucleotidesBPHYT_RS27050 and BPHYT_RS27055 are separated by 90 nucleotidesBPHYT_RS27055 and BPHYT_RS27060 are separated by 347 nucleotides BPHYT_RS27040: BPHYT_RS27040 - permease, at 1,642,128 to 1,642,679 _RS27040 BPHYT_RS27045: BPHYT_RS27045 - hypothetical protein, at 1,642,812 to 1,643,153 _RS27045 BPHYT_RS27050: BPHYT_RS27050 - DNA topoisomerase, at 1,643,363 to 1,644,682 _RS27050 BPHYT_RS27055: BPHYT_RS27055 - hypothetical protein, at 1,644,773 to 1,645,066 _RS27055 BPHYT_RS27060: BPHYT_RS27060 - photosystem reaction center subunit H, at 1,645,414 to 1,645,911 _RS27060 Position (kb) 1643 1644 1645Strain fitness (log2 ratio) -2 -1 0 1at 1642.462 kb on + strand, within BPHYT_RS27040at 1642.644 kb on + strandat 1642.683 kb on + strandat 1643.675 kb on + strand, within BPHYT_RS27050at 1644.080 kb on - strand, within BPHYT_RS27050at 1644.116 kb on + strand, within BPHYT_RS27050at 1644.308 kb on + strand, within BPHYT_RS27050at 1644.787 kb on + strandat 1645.082 kb on + strandat 1645.142 kb on + strandat 1645.142 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1
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1,642,462 + BPHYT_RS27040 0.61 -0.2
1,642,644 + -1.2
1,642,683 + -1.4
1,643,675 + BPHYT_RS27050 0.24 +0.0
1,644,080 - BPHYT_RS27050 0.54 +1.6
1,644,116 + BPHYT_RS27050 0.57 -1.5
1,644,308 + BPHYT_RS27050 0.72 -0.8
1,644,787 + -1.0
1,645,082 + -0.2
1,645,142 + +0.4
1,645,142 + -1.9

Or see this region's nucleotide sequence