Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24345 and BPHYT_RS24350 are separated by 45 nucleotides BPHYT_RS24350 and BPHYT_RS24355 are separated by 99 nucleotides BPHYT_RS24355 and BPHYT_RS24360 are separated by 294 nucleotides
BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978
_RS24345
BPHYT_RS24350: BPHYT_RS24350 - hypothetical protein, at 1,029,024 to 1,029,656
_RS24350
BPHYT_RS24355: BPHYT_RS24355 - hypothetical protein, at 1,029,756 to 1,030,007
_RS24355
BPHYT_RS24360: BPHYT_RS24360 - potassium transporter TrkA, at 1,030,302 to 1,031,378
_RS24360
Position (kb)
1029
1030 Strain fitness (log2 ratio)
-2
-1
0
1 at 1029.060 kb on + strand at 1029.060 kb on + strand at 1029.330 kb on + strand, within BPHYT_RS24350 at 1029.585 kb on + strand, within BPHYT_RS24350 at 1029.586 kb on - strand, within BPHYT_RS24350 at 1029.768 kb on + strand at 1029.996 kb on - strand at 1029.996 kb on - strand at 1030.053 kb on + strand at 1030.054 kb on - strand at 1030.054 kb on - strand at 1030.054 kb on - strand at 1030.122 kb on + strand at 1030.237 kb on + strand at 1030.459 kb on - strand, within BPHYT_RS24360 at 1030.522 kb on - strand, within BPHYT_RS24360 at 1030.522 kb on - strand, within BPHYT_RS24360 at 1030.656 kb on + strand, within BPHYT_RS24360
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,029,060 + -1.3 1,029,060 + +1.3 1,029,330 + BPHYT_RS24350 0.48 -0.7 1,029,585 + BPHYT_RS24350 0.89 -0.5 1,029,586 - BPHYT_RS24350 0.89 -1.0 1,029,768 + -0.1 1,029,996 - -0.0 1,029,996 - -0.2 1,030,053 + -1.0 1,030,054 - -0.2 1,030,054 - +0.1 1,030,054 - +0.7 1,030,122 + -0.3 1,030,237 + -1.8 1,030,459 - BPHYT_RS24360 0.15 -2.4 1,030,522 - BPHYT_RS24360 0.20 +0.4 1,030,522 - BPHYT_RS24360 0.20 -1.8 1,030,656 + BPHYT_RS24360 0.33 -0.7
Or see this region's nucleotide sequence