Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24335 and BPHYT_RS24340 are separated by 2 nucleotides BPHYT_RS24340 and BPHYT_RS24345 are separated by 282 nucleotides BPHYT_RS24345 and BPHYT_RS24350 are separated by 45 nucleotides BPHYT_RS24350 and BPHYT_RS24355 are separated by 99 nucleotides
BPHYT_RS24335: BPHYT_RS24335 - toxin HipA, at 1,025,758 to 1,027,098
_RS24335
BPHYT_RS24340: BPHYT_RS24340 - XRE family transcriptional regulator, at 1,027,101 to 1,027,355
_RS24340
BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978
_RS24345
BPHYT_RS24350: BPHYT_RS24350 - hypothetical protein, at 1,029,024 to 1,029,656
_RS24350
BPHYT_RS24355: BPHYT_RS24355 - hypothetical protein, at 1,029,756 to 1,030,007
_RS24355
Position (kb)
1027
1028
1029 Strain fitness (log2 ratio)
-1
0
1 at 1026.840 kb on - strand, within BPHYT_RS24335 at 1026.999 kb on - strand at 1027.483 kb on - strand at 1027.502 kb on - strand at 1027.622 kb on - strand at 1027.622 kb on - strand at 1027.749 kb on + strand at 1027.752 kb on + strand at 1027.752 kb on + strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1029.060 kb on + strand at 1029.060 kb on + strand at 1029.330 kb on + strand, within BPHYT_RS24350 at 1029.585 kb on + strand, within BPHYT_RS24350 at 1029.586 kb on - strand, within BPHYT_RS24350 at 1029.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,026,840 - BPHYT_RS24335 0.81 +0.2 1,026,999 - +0.1 1,027,483 - +0.2 1,027,502 - +0.3 1,027,622 - +0.2 1,027,622 - -0.7 1,027,749 + +0.5 1,027,752 + -0.9 1,027,752 + -0.7 1,027,753 - +0.1 1,027,753 - -0.4 1,027,753 - -0.5 1,027,753 - -0.6 1,029,060 + -1.3 1,029,060 + +1.3 1,029,330 + BPHYT_RS24350 0.48 -0.7 1,029,585 + BPHYT_RS24350 0.89 -0.5 1,029,586 - BPHYT_RS24350 0.89 -1.0 1,029,768 + -0.1
Or see this region's nucleotide sequence