Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23060 and BPHYT_RS23065 are separated by 122 nucleotides BPHYT_RS23065 and BPHYT_RS23070 are separated by 40 nucleotides
BPHYT_RS23060: BPHYT_RS23060 - 3-isopropylmalate dehydratase large subunit, at 734,683 to 736,650
_RS23060
BPHYT_RS23065: BPHYT_RS23065 - MFS transporter, at 736,773 to 738,239
_RS23065
BPHYT_RS23070: BPHYT_RS23070 - hypothetical protein, at 738,280 to 740,211
_RS23070
Position (kb) 736
737
738
739 Strain fitness (log2 ratio) -1
0
1
2
3 at 735.800 kb on - strand, within BPHYT_RS23060 at 736.130 kb on - strand, within BPHYT_RS23060 at 736.332 kb on + strand, within BPHYT_RS23060 at 736.332 kb on + strand, within BPHYT_RS23060 at 736.705 kb on - strand at 736.738 kb on + strand at 736.993 kb on - strand, within BPHYT_RS23065 at 737.102 kb on + strand, within BPHYT_RS23065 at 737.102 kb on + strand, within BPHYT_RS23065 at 737.163 kb on + strand, within BPHYT_RS23065 at 737.790 kb on + strand, within BPHYT_RS23065 at 737.893 kb on + strand, within BPHYT_RS23065 at 737.897 kb on + strand, within BPHYT_RS23065 at 738.237 kb on + strand at 738.271 kb on + strand at 738.303 kb on + strand at 738.304 kb on - strand at 738.304 kb on - strand at 738.652 kb on - strand, within BPHYT_RS23070 at 738.806 kb on + strand, within BPHYT_RS23070 at 738.806 kb on + strand, within BPHYT_RS23070 at 738.807 kb on - strand, within BPHYT_RS23070 at 739.050 kb on + strand, within BPHYT_RS23070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 735,800 - BPHYT_RS23060 0.57 +2.3 736,130 - BPHYT_RS23060 0.74 -0.0 736,332 + BPHYT_RS23060 0.84 -0.0 736,332 + BPHYT_RS23060 0.84 -0.2 736,705 - +1.3 736,738 + +0.5 736,993 - BPHYT_RS23065 0.15 +0.3 737,102 + BPHYT_RS23065 0.22 +3.0 737,102 + BPHYT_RS23065 0.22 +1.5 737,163 + BPHYT_RS23065 0.27 -1.5 737,790 + BPHYT_RS23065 0.69 +1.7 737,893 + BPHYT_RS23065 0.76 +0.4 737,897 + BPHYT_RS23065 0.77 +1.2 738,237 + -1.0 738,271 + +0.1 738,303 + +1.9 738,304 - +0.7 738,304 - +0.7 738,652 - BPHYT_RS23070 0.19 +0.7 738,806 + BPHYT_RS23070 0.27 -0.7 738,806 + BPHYT_RS23070 0.27 +1.3 738,807 - BPHYT_RS23070 0.27 -0.1 739,050 + BPHYT_RS23070 0.40 -0.1
Or see this region's nucleotide sequence