Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22050 and BPHYT_RS22055 are separated by 181 nucleotides BPHYT_RS22055 and BPHYT_RS22060 are separated by 923 nucleotides
BPHYT_RS22050: BPHYT_RS22050 - peptide ABC transporter substrate-binding protein, at 501,857 to 503,473
_RS22050
BPHYT_RS22055: BPHYT_RS22055 - DNA-binding protein, at 503,655 to 504,227
_RS22055
BPHYT_RS22060: BPHYT_RS22060 - amino acid transporter, at 505,151 to 506,692
_RS22060
Position (kb) 503
504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 502.836 kb on + strand, within BPHYT_RS22050 at 503.118 kb on + strand, within BPHYT_RS22050 at 503.640 kb on + strand at 504.063 kb on - strand, within BPHYT_RS22055 at 504.193 kb on - strand at 504.263 kb on + strand at 504.264 kb on - strand at 504.266 kb on - strand at 504.266 kb on - strand at 504.266 kb on - strand at 504.270 kb on - strand at 504.270 kb on - strand at 504.274 kb on - strand at 504.358 kb on - strand at 504.358 kb on - strand at 504.441 kb on - strand at 504.494 kb on - strand at 504.544 kb on - strand at 504.812 kb on + strand at 504.813 kb on - strand at 504.813 kb on - strand at 504.813 kb on - strand at 505.145 kb on + strand at 505.146 kb on - strand at 505.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 502,836 + BPHYT_RS22050 0.61 -1.3 503,118 + BPHYT_RS22050 0.78 +0.3 503,640 + +2.3 504,063 - BPHYT_RS22055 0.71 +0.6 504,193 - +0.0 504,263 + +1.8 504,264 - -1.8 504,266 - +0.3 504,266 - +0.2 504,266 - -0.4 504,270 - +1.5 504,270 - -3.3 504,274 - +0.0 504,358 - +2.0 504,358 - +0.1 504,441 - -0.3 504,494 - -0.7 504,544 - -0.2 504,812 + +0.3 504,813 - -0.4 504,813 - +0.0 504,813 - -0.1 505,145 + -1.3 505,146 - +0.9 505,146 - +1.9
Or see this region's nucleotide sequence