Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21540 and BPHYT_RS21545 are separated by 92 nucleotides BPHYT_RS21545 and BPHYT_RS21550 are separated by 168 nucleotides BPHYT_RS21550 and BPHYT_RS21555 are separated by 210 nucleotides
BPHYT_RS21540: BPHYT_RS21540 - 4Fe-4S ferredoxin, at 392,317 to 392,613
_RS21540
BPHYT_RS21545: BPHYT_RS21545 - SAM-dependent methyltransferase, at 392,706 to 393,422
_RS21545
BPHYT_RS21550: BPHYT_RS21550 - hypothetical protein, at 393,591 to 394,109
_RS21550
BPHYT_RS21555: BPHYT_RS21555 - beta alanine--pyruvate aminotransferase, at 394,320 to 395,648
_RS21555
Position (kb) 393
394
395 Strain fitness (log2 ratio) -2
-1
0
1
2 at 392.627 kb on + strand at 392.669 kb on - strand at 392.705 kb on + strand at 392.829 kb on + strand, within BPHYT_RS21545 at 392.964 kb on - strand, within BPHYT_RS21545 at 393.095 kb on + strand, within BPHYT_RS21545 at 393.144 kb on + strand, within BPHYT_RS21545 at 393.144 kb on + strand, within BPHYT_RS21545 at 393.224 kb on - strand, within BPHYT_RS21545 at 393.238 kb on - strand, within BPHYT_RS21545 at 393.284 kb on + strand, within BPHYT_RS21545 at 393.285 kb on - strand, within BPHYT_RS21545 at 393.306 kb on + strand, within BPHYT_RS21545 at 393.306 kb on + strand, within BPHYT_RS21545 at 393.306 kb on + strand, within BPHYT_RS21545 at 393.333 kb on + strand, within BPHYT_RS21545 at 394.113 kb on - strand at 394.220 kb on + strand at 394.241 kb on + strand at 394.321 kb on + strand at 394.322 kb on - strand at 394.633 kb on + strand, within BPHYT_RS21555 at 394.684 kb on + strand, within BPHYT_RS21555 at 394.837 kb on + strand, within BPHYT_RS21555
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 392,627 + -2.0 392,669 - -0.9 392,705 + +0.5 392,829 + BPHYT_RS21545 0.17 -1.9 392,964 - BPHYT_RS21545 0.36 -1.1 393,095 + BPHYT_RS21545 0.54 -0.6 393,144 + BPHYT_RS21545 0.61 +0.7 393,144 + BPHYT_RS21545 0.61 -1.3 393,224 - BPHYT_RS21545 0.72 -1.5 393,238 - BPHYT_RS21545 0.74 -1.2 393,284 + BPHYT_RS21545 0.81 +2.4 393,285 - BPHYT_RS21545 0.81 -1.3 393,306 + BPHYT_RS21545 0.84 +1.9 393,306 + BPHYT_RS21545 0.84 -0.9 393,306 + BPHYT_RS21545 0.84 -0.4 393,333 + BPHYT_RS21545 0.87 -0.7 394,113 - +1.2 394,220 + -0.3 394,241 + +0.0 394,321 + -0.8 394,322 - +1.0 394,633 + BPHYT_RS21555 0.24 -1.0 394,684 + BPHYT_RS21555 0.27 -1.1 394,837 + BPHYT_RS21555 0.39 +0.3
Or see this region's nucleotide sequence