Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21145 and BPHYT_RS21150 are separated by 67 nucleotides BPHYT_RS21150 and BPHYT_RS21155 are separated by 10 nucleotides BPHYT_RS21155 and BPHYT_RS21160 overlap by 4 nucleotides
BPHYT_RS21145: BPHYT_RS21145 - allantoate amidohydrolase, at 304,405 to 305,643
_RS21145
BPHYT_RS21150: BPHYT_RS21150 - NAD(P) transhydrogenase subunit alpha, at 305,711 to 306,829
_RS21150
BPHYT_RS21155: BPHYT_RS21155 - NAD(P) transhydrogenase, at 306,840 to 307,196
_RS21155
BPHYT_RS21160: BPHYT_RS21160 - NAD(P) transhydrogenase subunit beta, at 307,193 to 308,656
_RS21160
Position (kb) 305
306
307 Strain fitness (log2 ratio) -2
-1
0
1
2 at 304.773 kb on + strand, within BPHYT_RS21145 at 305.724 kb on + strand at 305.786 kb on + strand at 305.858 kb on + strand, within BPHYT_RS21150 at 305.859 kb on - strand, within BPHYT_RS21150 at 306.122 kb on + strand, within BPHYT_RS21150 at 306.123 kb on - strand, within BPHYT_RS21150 at 306.699 kb on - strand, within BPHYT_RS21150 at 306.847 kb on + strand at 306.883 kb on + strand, within BPHYT_RS21155 at 306.894 kb on + strand, within BPHYT_RS21155 at 307.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 304,773 + BPHYT_RS21145 0.30 -0.2 305,724 + +1.9 305,786 + -2.3 305,858 + BPHYT_RS21150 0.13 -2.3 305,859 - BPHYT_RS21150 0.13 -1.5 306,122 + BPHYT_RS21150 0.37 +0.4 306,123 - BPHYT_RS21150 0.37 -0.6 306,699 - BPHYT_RS21150 0.88 -0.9 306,847 + -0.1 306,883 + BPHYT_RS21155 0.12 -0.6 306,894 + BPHYT_RS21155 0.15 -1.0 307,229 + -0.6
Or see this region's nucleotide sequence