Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21020

Experiment: 24hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21015 and BPHYT_RS21020 are separated by 272 nucleotidesBPHYT_RS21020 and BPHYT_RS21025 are separated by 329 nucleotides BPHYT_RS21015: BPHYT_RS21015 - transcriptional regulator, at 273,265 to 274,356 _RS21015 BPHYT_RS21020: BPHYT_RS21020 - branched-chain amino acid ABC transporter substrate-binding protein, at 274,629 to 275,753 _RS21020 BPHYT_RS21025: BPHYT_RS21025 - cation-binding protein, at 276,083 to 276,619 _RS21025 Position (kb) 274 275 276Strain fitness (log2 ratio) -1 0 1 2 3at 274.582 kb on + strandat 274.741 kb on - strandat 274.928 kb on + strand, within BPHYT_RS21020at 274.965 kb on + strand, within BPHYT_RS21020at 274.966 kb on - strand, within BPHYT_RS21020at 275.092 kb on - strand, within BPHYT_RS21020at 275.544 kb on + strand, within BPHYT_RS21020at 275.709 kb on + strandat 275.710 kb on - strandat 275.710 kb on - strandat 276.347 kb on + strand, within BPHYT_RS21025at 276.734 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1
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274,582 + -0.6
274,741 - -0.8
274,928 + BPHYT_RS21020 0.27 -0.5
274,965 + BPHYT_RS21020 0.30 +0.3
274,966 - BPHYT_RS21020 0.30 -0.2
275,092 - BPHYT_RS21020 0.41 -0.3
275,544 + BPHYT_RS21020 0.81 -1.1
275,709 + -0.1
275,710 - -1.3
275,710 - +0.1
276,347 + BPHYT_RS21025 0.49 -1.7
276,734 - +2.9

Or see this region's nucleotide sequence